Artículo
Use of the Leptospira sp. ligB C-terminus coding region as a diagnostic tool of animal leptospirosis
Saraullo, Vanina Rosa
; Grune Loffler, Sylvia
; Florin Christensen, Monica; Watanabe, Olivia
; Hamer, Micaela
; Martinez, Mara; Brihuega, Bibiana
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Fecha de publicación:
10/2021
Editorial:
Elsevier
Revista:
Comparative Immunology, Microbiology and Infectious Diseases
ISSN:
0147-9571
Idioma:
Inglés
Tipo de recurso:
Artículo publicado
Clasificación temática:
Resumen
Leptospirosis is the most widespread zoonosis worldwide, and it can cause reproductive failures in livestock, while in humans may vary from a mild fever to multi-organ failure and death. Due to this, in this study, we evaluated the usefulness of the segment encoding LigB C-terminus region, only present in pathogenic as target for a diagnostic PCR. This new PCR yielded a 100 % positivity for pathogenic Leptospira species and no cross-reactivity was found with intermediate or non-pathogenic species, or with other microorganisms, demostrating its high analytical specificity. The estimated analytical sensitivity was higher in serum samples than in blood or urine samples (6−9 × 102 lept/mL and 6−9 × 105 and 6−9 × 106 lept/mL, respectively). Multiple sequence alignment of the target region from different pathogenic Leptospira species confirmed that this gene region is highly conserved among these species, with few single nucleotide polymorphisms. The ligb-ct PCR here developed appears as a useful tool for the molecular diagnosis of leptospirosis.
Palabras clave:
DIAGNOSTIC METHODS
,
INFECTIOUS DISEASES
,
LEPTOSPIRA
,
LEPTOSPIROSIS
,
LIGB GENE
,
PCR
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Colecciones
Articulos (IPVET)
Articulos de INSTITUTO DE PATOBIOLOGIA VETERINARIA
Articulos de INSTITUTO DE PATOBIOLOGIA VETERINARIA
Citación
Saraullo, Vanina Rosa; Grune Loffler, Sylvia; Florin Christensen, Monica; Watanabe, Olivia; Hamer, Micaela; et al.; Use of the Leptospira sp. ligB C-terminus coding region as a diagnostic tool of animal leptospirosis; Elsevier; Comparative Immunology, Microbiology and Infectious Diseases; 78; 101689; 10-2021; 1-5
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