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Artículo

Use of the Leptospira sp. ligB C-terminus coding region as a diagnostic tool of animal leptospirosis

Saraullo, Vanina RosaIcon ; Grune Loffler, SylviaIcon ; Florin Christensen, Monica; Watanabe, OliviaIcon ; Hamer, MicaelaIcon ; Martinez, Mara; Brihuega, Bibiana
Fecha de publicación: 10/2021
Editorial: Elsevier
Revista: Comparative Immunology, Microbiology and Infectious Diseases
ISSN: 0147-9571
Idioma: Inglés
Tipo de recurso: Artículo publicado
Clasificación temática:
Ciencias Veterinarias

Resumen

Leptospirosis is the most widespread zoonosis worldwide, and it can cause reproductive failures in livestock, while in humans may vary from a mild fever to multi-organ failure and death. Due to this, in this study, we evaluated the usefulness of the segment encoding LigB C-terminus region, only present in pathogenic as target for a diagnostic PCR. This new PCR yielded a 100 % positivity for pathogenic Leptospira species and no cross-reactivity was found with intermediate or non-pathogenic species, or with other microorganisms, demostrating its high analytical specificity. The estimated analytical sensitivity was higher in serum samples than in blood or urine samples (6−9 × 102 lept/mL and 6−9 × 105 and 6−9 × 106 lept/mL, respectively). Multiple sequence alignment of the target region from different pathogenic Leptospira species confirmed that this gene region is highly conserved among these species, with few single nucleotide polymorphisms. The ligb-ct PCR here developed appears as a useful tool for the molecular diagnosis of leptospirosis.
Palabras clave: DIAGNOSTIC METHODS , INFECTIOUS DISEASES , LEPTOSPIRA , LEPTOSPIROSIS , LIGB GENE , PCR
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info:eu-repo/semantics/restrictedAccess Excepto donde se diga explícitamente, este item se publica bajo la siguiente descripción: Creative Commons Attribution-NonCommercial-ShareAlike 2.5 Unported (CC BY-NC-SA 2.5)
Identificadores
URI: http://hdl.handle.net/11336/182993
DOI: http://dx.doi.org/10.1016/j.cimid.2021.101689
URL: https://www.sciencedirect.com/science/article/abs/pii/S0147957121000813
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Articulos (IPVET)
Articulos de INSTITUTO DE PATOBIOLOGIA VETERINARIA
Citación
Saraullo, Vanina Rosa; Grune Loffler, Sylvia; Florin Christensen, Monica; Watanabe, Olivia; Hamer, Micaela; et al.; Use of the Leptospira sp. ligB C-terminus coding region as a diagnostic tool of animal leptospirosis; Elsevier; Comparative Immunology, Microbiology and Infectious Diseases; 78; 101689; 10-2021; 1-5
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