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dc.contributor.author
Aguirre, Matías Ezequiel
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Marino, Ignacio
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Bozzano, Patricia Beatriz
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Feldhoff, Armin
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Grela, Maria Alejandra
dc.date.available
2023-01-02T18:37:11Z
dc.date.issued
2019-07
dc.identifier.citation
Aguirre, Matías Ezequiel; Marino, Ignacio; Bozzano, Patricia Beatriz; Feldhoff, Armin; Grela, Maria Alejandra; Magnetic ZIF-8 as a stable support for biomolecules via adsorption in aqueous buffered solutions at pH = 7; Elsevier Science; Inorganic Chemistry Communications; 105; 7-2019; 225-229
dc.identifier.issn
1387-7003
dc.identifier.uri
http://hdl.handle.net/11336/182961
dc.description.abstract
Metal-organic frameworks (MOFs) have been recently proposed as supports for biomolecules immobilization, particularly for enzymes. However, their stability under the high ionic strength aqueous solutions required for practical applications of these biocatalysts have not been evaluated. Here, it is shown that surface modifications and a careful analysis and selection of the buffer components must be accomplished to ensure the resistance of the MOF to hydrolysis, even for the previously assumed robust Zeolitic Imidazole Frameworks (ZIFs). By taking into account these considerations, and introducing polyvinylpyrrolidone (PVP) as a surfactant in the synthetic process, a resistant and stable magnetic MOF (Fe 3 O 4 @ZIF-8/PVP) was obtained. Further, the loading capacity of the stabilized synthetized structures for the immobilization of a model protein, such as bovine serum albumin (BSA), was analyzed. It was found that physical interaction provides an efficient and suitable method to immobilize this biomolecule without evidence of leaching.
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application/pdf
dc.language.iso
eng
dc.publisher
Elsevier Science
dc.rights
info:eu-repo/semantics/restrictedAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
ADSORPTION
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BIOMOLECULE SUPPORT
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BUFFERED MEDIA
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METAL-ORGANIC FRAMEWORKS
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ZIF-8 STABILITY
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Otras Ciencias Químicas
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Ciencias Químicas
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CIENCIAS NATURALES Y EXACTAS
dc.title
Magnetic ZIF-8 as a stable support for biomolecules via adsorption in aqueous buffered solutions at pH = 7
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2022-11-17T17:36:36Z
dc.journal.volume
105
dc.journal.pagination
225-229
dc.journal.pais
Países Bajos
dc.journal.ciudad
Amsterdam
dc.description.fil
Fil: Aguirre, Matías Ezequiel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Físicas de Mar del Plata. Grupo de Investigación del Departamento de Química de la Unmdp | Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones Físicas de Mar del Plata. Grupo de Investigación del Departamento de Química de la Unmdp; Argentina
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Fil: Marino, Ignacio. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Departamento de Química; Argentina
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Fil: Bozzano, Patricia Beatriz. Comisión Nacional de Energía Atómica. Centro Atómico Constituyentes; Argentina. Universidad Nacional de San Martín. Instituto Sabato; Argentina
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Fil: Feldhoff, Armin. Leibniz Universitat Hannover; Alemania
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Fil: Grela, Maria Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Físicas de Mar del Plata. Grupo de Investigación del Departamento de Química de la Unmdp | Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones Físicas de Mar del Plata. Grupo de Investigación del Departamento de Química de la Unmdp; Argentina
dc.journal.title
Inorganic Chemistry Communications
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://linkinghub.elsevier.com/retrieve/pii/S1387700319300954
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info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1016/j.inoche.2019.05.013
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