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dc.contributor.author
Potuschak, Thomas
dc.contributor.author
Palatnik, Javier Fernando
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dc.contributor.author
Schommer, Carla
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dc.contributor.author
Sierro, Nicolas
dc.contributor.author
Ivanov, Nicolai
dc.contributor.author
Kwon, Yerim
dc.contributor.author
Genschik, Pascal
dc.contributor.author
Daviere, Jean-Michel
dc.contributor.author
Otten, Leoon
dc.date.available
2022-12-30T02:05:16Z
dc.date.issued
2020-01
dc.identifier.citation
Potuschak, Thomas; Palatnik, Javier Fernando; Schommer, Carla; Sierro, Nicolas; Ivanov, Nicolai; et al.; Inhibition of Arabidopsis thaliana CIN-like TCP transcription factors by Agrobacterium T-DNA-encoded 6B proteins; Wiley Blackwell Publishing, Inc; Plant Journal; 1-2020
dc.identifier.issn
0960-7412
dc.identifier.uri
http://hdl.handle.net/11336/182882
dc.description.abstract
Agrobacterium T-DNA-encoded 6B proteins cause remarkable growth effects in plants. Nicotiana otophora carries two cellular T-DNAs with three slightly divergent 6b genes (TE-1-6b-L, TE-1-6b-R and TE-2-6b) originating from a natural transformation event. In Arabidopsis thaliana, expression of 2×35S:TE-2-6b, but not 2×35S:TE-1-6b-L or 2×35S:TE-1-6b-R, led to plants with crinkly leaves, which strongly resembled mutants of the miR319a/TCP module. This module is composed of MIR319A and five CIN-like TCP (TEOSINTHE BRANCHED1, CYCLOIDEA and PROLIFERATING CELL NUCLEAR ANTIGEN BINDING FACTOR) genes (TCP2, TCP3, TCP4, TCP10 and TCP24) targeted by miR319a. The CIN-like TCP genes encode transcription factors and are required for cell division arrest at leaf margins during development. MIR319A overexpression causes excessive growth and crinkly leaves. TE-2-6b plants did not show increased miR319a levels, but the mRNA levels of the TCP4 target gene LOX2 were decreased, as in jaw-D plants. Co-expression of green fluorescent protein (GFP)-tagged TCPs with native or red fluorescent protein (RFP)-tagged TE-6B proteins led to an increase in TCP protein levels and formation of numerous cytoplasmic dots containing 6B and TCP proteins. Yeast double-hybrid experiments confirmed 6B/TCP binding and showed that TE-1-6B-L and TE-1-6B-R bind a smaller set of TCP proteins than TE-2-6B. A single nucleotide mutation in TE-1-6B-R enlarged its TCP-binding repertoire to that of TE-2-6B and caused a crinkly phenotype in Arabidopsis. Deletion analysis showed that TE-2-6B targets the TCP4 DNA-binding domain and directly interferes with transcriptional activation. Taken together, these results provide detailed insights into the mechanism of action of the N. otophora TE-encoded 6b genes.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Wiley Blackwell Publishing, Inc
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dc.rights
info:eu-repo/semantics/openAccess
dc.rights.uri
https://creativecommons.org/licenses/by/2.5/ar/
dc.subject
6B ONCOGENE
dc.subject
JAW-D PHENOTYPE
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NATURAL TRANSFORMANT
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NICOTIANA OTOPHORA
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TCP GENES
dc.subject.classification
Biología del Desarrollo
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dc.subject.classification
Ciencias Biológicas
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dc.subject.classification
CIENCIAS NATURALES Y EXACTAS
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dc.title
Inhibition of Arabidopsis thaliana CIN-like TCP transcription factors by Agrobacterium T-DNA-encoded 6B proteins
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2021-09-06T21:05:21Z
dc.journal.pais
Reino Unido
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dc.journal.ciudad
Londres
dc.description.fil
Fil: Potuschak, Thomas. Centre National de la Recherche Scientifique. Institut de Biologie Moléculaire des Plantes; Francia
dc.description.fil
Fil: Palatnik, Javier Fernando. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Biología Molecular y Celular de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario; Argentina
dc.description.fil
Fil: Schommer, Carla. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Biología Molecular y Celular de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario; Argentina
dc.description.fil
Fil: Sierro, Nicolas. Pmi R&d, Philip Morris Products S. A.; Suiza
dc.description.fil
Fil: Ivanov, Nicolai. Pmi R&d, Philip Morris Products S. A.; Suiza
dc.description.fil
Fil: Kwon, Yerim. Centre National de la Recherche Scientifique. Institut de Biologie Moléculaire des Plantes; Francia
dc.description.fil
Fil: Genschik, Pascal. Centre National de la Recherche Scientifique. Institut de Biologie Moléculaire des Plantes; Francia
dc.description.fil
Fil: Daviere, Jean-Michel. Centre National de la Recherche Scientifique. Institut de Biologie Moléculaire des Plantes; Francia
dc.description.fil
Fil: Otten, Leoon. Centre National de la Recherche Scientifique. Institut de Biologie Moléculaire des Plantes; Francia
dc.journal.title
Plant Journal
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dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1111/tpj.14591
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://onlinelibrary.wiley.com/doi/10.1111/tpj.14591
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