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dc.contributor.author
Wu, Zhenyong
dc.contributor.author
Bello, Oscar Daniel
dc.contributor.author
Thiyagarajan, Sathish
dc.contributor.author
Auclair, Sarah Marie
dc.contributor.author
Vennekate, Wensi
dc.contributor.author
Krishnakumar, Shyam S
dc.contributor.author
O'Shaughnessy, Ben
dc.contributor.author
Karatekin, Erdem
dc.date.available
2022-12-29T17:10:22Z
dc.date.issued
2017-03
dc.identifier.citation
Wu, Zhenyong; Bello, Oscar Daniel; Thiyagarajan, Sathish; Auclair, Sarah Marie; Vennekate, Wensi; et al.; Dilation of fusion pores by crowding of SNARE proteins; eLife Sciences Publications; eLife; 6; e22964; 3-2017; 1-26
dc.identifier.uri
http://hdl.handle.net/11336/182835
dc.description.abstract
Hormones and neurotransmitters are released through fluctuating exocytotic fusion pores that can flicker open and shut multiple times. Cargo release and vesicle recycling depend on the fate of the pore, which may reseal or dilate irreversibly. Pore nucleation requires zippering between vesicle-associated v-SNAREs and target membrane t-SNAREs, but the mechanisms governing the subsequent pore dilation are not understood. Here, we probed the dilation of single fusion pores using v-SNARE-reconstituted ~23-nm-diameter discoidal nanolipoprotein particles (vNLPs) as fusion partners with cells ectopically expressing cognate, ’flipped’ t-SNAREs. Pore nucleation required a minimum of two v-SNAREs per NLP face, and further increases in v-SNARE copy numbers did not affect nucleation rate. By contrast, the probability of pore dilation increased with increasing v-SNARE copies and was far from saturating at 15 v-SNARE copies per face, the NLP capacity. Our experimental and computational results suggest that SNARE availability may be pivotal in determining whether neurotransmitters or hormones are released through a transient (’kiss and run’) or an irreversibly dilating pore (full fusion).
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
eLife Sciences Publications
dc.rights
info:eu-repo/semantics/openAccess
dc.rights.uri
https://creativecommons.org/licenses/by/2.5/ar/
dc.subject
E. COLI
dc.subject
SNARE PROTEINS
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BIOPHYSICS
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CELL BIOLOGY
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EXOCYTOSIS
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FUSION PORE
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MEMBRANE FUSION
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PROTEIN CROWDING
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STRUCTURAL BIOLOGY
dc.subject.classification
Biología Celular, Microbiología
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Ciencias Biológicas
dc.subject.classification
CIENCIAS NATURALES Y EXACTAS
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Bioquímica y Biología Molecular
dc.subject.classification
Ciencias Biológicas
dc.subject.classification
CIENCIAS NATURALES Y EXACTAS
dc.subject.classification
Biofísica
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Ciencias Biológicas
dc.subject.classification
CIENCIAS NATURALES Y EXACTAS
dc.title
Dilation of fusion pores by crowding of SNARE proteins
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2022-12-27T18:07:48Z
dc.identifier.eissn
2050-084X
dc.journal.volume
6
dc.journal.number
e22964
dc.journal.pagination
1-26
dc.journal.pais
Reino Unido
dc.journal.ciudad
Londres
dc.description.fil
Fil: Wu, Zhenyong. University of Yale; Estados Unidos. University of Yale. School of Medicine; Estados Unidos
dc.description.fil
Fil: Bello, Oscar Daniel. University of Yale. School of Medicine; Estados Unidos. University of Yale; Estados Unidos. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos. Universidad Nacional de Cuyo. Facultad de Ciencias Médicas. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos; Argentina
dc.description.fil
Fil: Thiyagarajan, Sathish. Columbia University; Estados Unidos
dc.description.fil
Fil: Auclair, Sarah Marie. University of Yale. School of Medicine; Estados Unidos. University of Yale; Estados Unidos
dc.description.fil
Fil: Vennekate, Wensi. University of Yale; Estados Unidos. University of Yale. School of Medicine; Estados Unidos
dc.description.fil
Fil: Krishnakumar, Shyam S. University of Yale; Estados Unidos. University of Yale. School of Medicine; Estados Unidos
dc.description.fil
Fil: O'Shaughnessy, Ben. Columbia University; Estados Unidos
dc.description.fil
Fil: Karatekin, Erdem. University of Yale; Estados Unidos. Universite Paris Descartes; Francia. University of Yale. School of Medicine; Estados Unidos
dc.journal.title
eLife
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://elifesciences.org/articles/22964
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.7554/eLife.22964
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