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dc.contributor.author
Wang, Jing
dc.contributor.author
Li, Feng
dc.contributor.author
Bello, Oscar Daniel
dc.contributor.author
Sindelar, Charles Vaughn
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Pincet, Frédéric
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Krishnakumar, Shyam S.
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Rothman, James E.
dc.date.available
2022-12-29T15:27:14Z
dc.date.issued
2017-08
dc.identifier.citation
Wang, Jing; Li, Feng; Bello, Oscar Daniel; Sindelar, Charles Vaughn; Pincet, Frédéric; et al.; Circular oligomerization is an intrinsic property of synaptotagmin; eLife Sciences Publications; eLife; 6; e27441; 8-2017; 1-17
dc.identifier.uri
http://hdl.handle.net/11336/182828
dc.description.abstract
Previously, we showed that synaptotagmin1 (Syt1) forms Ca2+-sensitive ring-like oligomers on membranes containing acidic lipids and proposed a potential role in regulating neurotransmitter release (Zanetti et al., 2016). Here, we report that Syt1 assembles into similar ring-like oligomers in solution when triggered by naturally occurring polyphosphates (PIP2 and ATP) and magnesium ions (Mg2+). These soluble Syt1 rings were observed by electron microscopy and independently demonstrated and quantified using fluorescence correlation spectroscopy. Oligomerization is triggered when polyphosphates bind to the polylysine patch in C2B domain and is stabilized by Mg2+, which neutralizes the Ca2+-binding aspartic acids that likely contribute to the C2B interface in the oligomer. Overall, our data show that ring-like polymerization is an intrinsic property of Syt1 with reasonable affinity that can be triggered by the vesicle docking C2B-PIP2 interaction and raise the possibility that Syt1 rings could pre-form on the synaptic vesicle to facilitate docking.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
eLife Sciences Publications
dc.rights
info:eu-repo/semantics/openAccess
dc.rights.uri
https://creativecommons.org/licenses/by/2.5/ar/
dc.subject
ELECTRON MICROSCOPY
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MEMBRANE FUSION
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NEUROSCIENCE
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NEUROTRANSMITTERS
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Biofísica
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Ciencias Biológicas
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CIENCIAS NATURALES Y EXACTAS
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Bioquímica y Biología Molecular
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Ciencias Biológicas
dc.subject.classification
CIENCIAS NATURALES Y EXACTAS
dc.title
Circular oligomerization is an intrinsic property of synaptotagmin
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2022-12-27T18:07:42Z
dc.identifier.eissn
2050-084X
dc.journal.volume
6
dc.journal.number
e27441
dc.journal.pagination
1-17
dc.journal.pais
Reino Unido
dc.journal.ciudad
Londres
dc.description.fil
Fil: Wang, Jing. University of Yale. School of Medicine; Estados Unidos
dc.description.fil
Fil: Li, Feng. University of Yale. School of Medicine; Estados Unidos
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Fil: Bello, Oscar Daniel. University of Yale. School of Medicine; Estados Unidos. University College London; Estados Unidos. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos. Universidad Nacional de Cuyo. Facultad de Ciencias Médicas. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos; Argentina
dc.description.fil
Fil: Sindelar, Charles Vaughn. University of Yale. School of Medicine; Estados Unidos
dc.description.fil
Fil: Pincet, Frédéric. University of Yale. School of Medicine; Estados Unidos. Universite de Paris VI; Francia. Université Paris Diderot - Paris 7; Francia
dc.description.fil
Fil: Krishnakumar, Shyam S.. University of Yale. School of Medicine; Estados Unidos. University College London; Estados Unidos
dc.description.fil
Fil: Rothman, James E.. University College London; Estados Unidos. University of Yale. School of Medicine; Estados Unidos
dc.journal.title
eLife
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://elifesciences.org/articles/27441
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.7554/eLife.27441
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