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dc.contributor.author
Noguera, Martín Ezequiel
dc.contributor.author
Roman, Ernesto Andres
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Rigal, Juan B.
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Cousido Siah, Alexandra
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Mitschler, André
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Podjarny, Alberto Daniel
dc.contributor.author
Santos, Javier
dc.date.available
2022-12-28T17:52:14Z
dc.date.issued
2015-06
dc.identifier.citation
Noguera, Martín Ezequiel; Roman, Ernesto Andres; Rigal, Juan B.; Cousido Siah, Alexandra; Mitschler, André; et al.; Structural characterization of metal binding to a cold-adapted frataxin; Springer; Journal of Biological Inorganic Chemistry; 20; 4; 6-2015; 653-664
dc.identifier.issn
0949-8257
dc.identifier.uri
http://hdl.handle.net/11336/182742
dc.description.abstract
Frataxin is an evolutionary conserved protein that participates in iron metabolism. Deficiency of this small protein in humans causes a severe neurodegenerative disease known as Friedreich's ataxia. A number of studies indicate that frataxin binds iron and regulates Fe-S cluster biosynthesis. Previous structural studies showed that metal binding occurs mainly in a region of high density of negative charge. However, a comprehensive characterization of the binding sites is required to gain further insights into the mechanistic details of frataxin function. In this work, we have solved the X-ray crystal structures of a cold-adapted frataxin from a psychrophilic bacterium in the presence of cobalt or europium ions. We have identified a number of metal-binding sites, mainly solvent exposed, several of which had not been observed in previous studies on mesophilic homologues. No major structural changes were detected upon metal binding, although the structures exhibit significant changes in crystallographic B-factors. The analysis of these B-factors, in combination with crystal packing and RMSD among structures, suggests the existence of localized changes in the internal motions. Based on these results, we propose that bacterial frataxins possess binding sites of moderate affinity for a quick capture and transfer of iron to other proteins and for the regulation of Fe-S cluster biosynthesis, modulating interactions with partner proteins.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Springer
dc.rights
info:eu-repo/semantics/openAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
CONFORMATIONAL DYNAMICS
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CYAY PROTEIN FAMILY
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EXTREMOPHILE
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IRON BINDING
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X-RAY DIFFRACTION
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Bioquímica y Biología Molecular
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Ciencias Biológicas
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CIENCIAS NATURALES Y EXACTAS
dc.title
Structural characterization of metal binding to a cold-adapted frataxin
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2022-12-28T14:01:01Z
dc.identifier.eissn
1432-1327
dc.journal.volume
20
dc.journal.number
4
dc.journal.pagination
653-664
dc.journal.pais
Alemania
dc.journal.ciudad
Berlin
dc.description.fil
Fil: Noguera, Martín Ezequiel. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Química y Físico-Química Biológicas "Prof. Alejandro C. Paladini". Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Química y Físico-Química Biológicas; Argentina
dc.description.fil
Fil: Roman, Ernesto Andres. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Química y Físico-Química Biológicas "Prof. Alejandro C. Paladini". Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Química y Físico-Química Biológicas; Argentina
dc.description.fil
Fil: Rigal, Juan B.. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Química y Físico-Química Biológicas "Prof. Alejandro C. Paladini". Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Química y Físico-Química Biológicas; Argentina
dc.description.fil
Fil: Cousido Siah, Alexandra. Centre National de la Recherche Scientifique. Igbmc; Francia. Université de Strasbourg; Francia
dc.description.fil
Fil: Mitschler, André. Centre National de la Recherche Scientifique. Igbmc; Francia. Université de Strasbourg; Francia
dc.description.fil
Fil: Podjarny, Alberto Daniel. Université de Strasbourg; Francia. Centre National de la Recherche Scientifique. Igbmc; Francia
dc.description.fil
Fil: Santos, Javier. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Química y Físico-Química Biológicas "Prof. Alejandro C. Paladini". Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Química y Físico-Química Biológicas; Argentina
dc.journal.title
Journal of Biological Inorganic Chemistry
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/http://link.springer.com/article/10.1007%2Fs00775-015-1251-9
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/https://doi.org/10.1007/s00775-015-1251-9
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