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dc.contributor.author
Amarelle, Vanesa
dc.contributor.author
Rosconi, Federico
dc.contributor.author
Lazaro Martinez, Juan Manuel
dc.contributor.author
Buldain, Graciela Yolanda
dc.contributor.author
Noya, Francisco
dc.contributor.author
O`Brian, Mark R.
dc.contributor.author
Fabiano, Elena
dc.date.available
2017-06-15T18:25:31Z
dc.date.issued
2016-04
dc.identifier.citation
Amarelle, Vanesa; Rosconi, Federico; Lazaro Martinez, Juan Manuel; Buldain, Graciela Yolanda; Noya, Francisco; et al.; HmuS and HmuQ of Ensifer/Sinorhizobium meliloti degrade heme in vitro and participate in heme metabolism in vivo; Springer; Biometals; 29; 2; 4-2016; 333-347
dc.identifier.issn
0966-0844
dc.identifier.uri
http://hdl.handle.net/11336/18264
dc.description.abstract
Ensifer meliloti is a nitrogen-fixing symbiont of the alfalfa legume able to use heme as an iron source. The transport mechanism involved in heme acquisition in E. meliloti has been identified and characterized, but the fate of heme once inside the cell is not known. In silico analysis of E. meliloti 1021 genome revealed no canonical heme oxygenases although two genes encoding putative heme degrading enzymes, smc01518 and hmuS, were identified. SMc01518 is similar to HmuQ of Bradyrhizobium japonicum, which is weakly homologous to the Staphylococcus aureus IsdG heme-degrading monooxygenase, whereas HmuS is homolog to Pseudomonas aeruginosa PhuS, a protein reported as a heme chaperone and as a heme degrading enzyme. Recombinant HmuQ and HmuS were able to bind hemin with a 1:1 stoichiometry and displayed a Kd value of 5 and 4 µM, respectively. HmuS degrades heme in vitro to the biliverdin isomers IX-β and IX-δ in an equimolar ratio. The HmuQ recombinant protein degrades heme to biliverdin IX-δ only. Additionally, in this work we demonstrate that humS and hmuQ gene expression is regulated by iron and heme in a RirA dependent manner and that both proteins are involved in heme metabolism in E. meliloti in vivo.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Springer
dc.rights
info:eu-repo/semantics/openAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
E. MELILOTI
dc.subject
HEME
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HEME-DEGRADATION
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HEME-OXYGENASE
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IRON METABOLISM
dc.subject
RHIZOBIA
dc.subject.classification
Bioquímica y Biología Molecular
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Ciencias Biológicas
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CIENCIAS NATURALES Y EXACTAS
dc.title
HmuS and HmuQ of Ensifer/Sinorhizobium meliloti degrade heme in vitro and participate in heme metabolism in vivo
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2017-06-15T17:36:49Z
dc.identifier.eissn
1572-8773
dc.journal.volume
29
dc.journal.number
2
dc.journal.pagination
333-347
dc.journal.pais
Alemania
dc.description.fil
Fil: Amarelle, Vanesa. Instituto de Investigaciones Biológicas "Clemente Estable"; Uruguay
dc.description.fil
Fil: Rosconi, Federico. Instituto de Investigaciones Biológicas "Clemente Estable"; Uruguay
dc.description.fil
Fil: Lazaro Martinez, Juan Manuel. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay; Argentina. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Química Orgánica; Argentina
dc.description.fil
Fil: Buldain, Graciela Yolanda. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay; Argentina. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Química Orgánica; Argentina
dc.description.fil
Fil: Noya, Francisco. Instituto de Investigaciones Biológicas "Clemente Estable"; Uruguay
dc.description.fil
Fil: O`Brian, Mark R.. State University of New York; Estados Unidos
dc.description.fil
Fil: Fabiano, Elena. Instituto de Investigaciones Biológicas "Clemente Estable"; Uruguay
dc.journal.title
Biometals
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://link.springer.com/article/10.1007%2Fs10534-016-9919-3
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1007/s10534-016-9919-3
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