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dc.contributor.author
Pino, María T. L.
dc.contributor.author
Verstraeten, Sandra Viviana
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dc.date.available
2017-06-15T18:16:49Z
dc.date.issued
2015-08
dc.identifier.citation
Pino, María T. L.; Verstraeten, Sandra Viviana; Tl(I) and Tl(III) alter the expression of EGF-dependent signals and cyclins required for pheochromocytoma (PC12) cell-cycle resumption and progression; Wiley; Journal of Applied Toxicology; 35; 8; 8-2015; 952-969
dc.identifier.issn
0260-437X
dc.identifier.uri
http://hdl.handle.net/11336/18263
dc.description.abstract
The effects of thallium [Tl(I) and Tl(III)] on the PC12 cell cycle were evaluated without (EGF−) or with (EGF+) media supplementation with epidermal growth factor (EGF). The following markers of cell-cycle phases were analyzed: cyclin D1 (G1); E2F-1, cyclin E and cytosolic p21 (G1→S transition); nuclear PCNA and cyclin A (S); and cyclin B1 (G2). The amount of cells in each phase and the activation of the signaling cascade triggered by EGF were also analyzed. Tl(I) and Tl(III) (5–100 μM) caused dissimilar effects on PC12 cell proliferation. In EGF− cells, Tl(I) increased the expression of G1→S transition markers and nuclear PCNA, without affecting cyclin A or cyclin B1. In addition to those, cyclin B1 was also increased in EGF+ cells. In EGF− cells, Tl(III) increased the expression of cyclin D1, all the G1→S and S phase markers and cyclin B1. In EGF+ cells, Tl(III) increased cyclin D1 expression and decreased all the markers of G1→S transition and the S phase. Even when these cations did not induce the activation of EGF receptor (EGFR) in EGF− cells, they promoted the phosphorylation of ERK1/2 and Akt. In the presence of EGF, the cations anticipated EGFR phosphorylation without affecting the kinetics of EGF-dependent ERK1/2 and Akt phosphorylation. Altogether, results indicate that Tl(I) promoted cell proliferation in both EGF− and EGF+ cells. In contrast, Tl(III) promoted the proliferation of EGF− cells but delayed it in EGF+ cells, which may be related to the toxic effects of this cation in PC12 cells.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Wiley
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dc.rights
info:eu-repo/semantics/openAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
Cell Cycle
dc.subject
Cell Signaling
dc.subject
Cyclins
dc.subject
Epidermal Growth Factor
dc.subject
Thallium
dc.subject.classification
Bioquímica y Biología Molecular
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dc.subject.classification
Ciencias Biológicas
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dc.subject.classification
CIENCIAS NATURALES Y EXACTAS
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dc.title
Tl(I) and Tl(III) alter the expression of EGF-dependent signals and cyclins required for pheochromocytoma (PC12) cell-cycle resumption and progression
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2017-06-15T17:36:20Z
dc.journal.volume
35
dc.journal.number
8
dc.journal.pagination
952-969
dc.journal.pais
Estados Unidos
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dc.journal.ciudad
Hoboken
dc.description.fil
Fil: Pino, María T. L.. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Química y Físico-Química Biológicas "Prof. Alejandro C. Paladini". Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Química y Físico-Química Biológicas; Argentina. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Química Biológica; Argentina
dc.description.fil
Fil: Verstraeten, Sandra Viviana. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Química Biológica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Química y Físico-Química Biológicas "Prof. Alejandro C. Paladini". Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Química y Físico-Química Biológicas; Argentina
dc.journal.title
Journal of Applied Toxicology
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dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/http://onlinelibrary.wiley.com/doi/10.1002/jat.3096/abstract
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1002/jat.3096
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