Artículo
From the notebook to recombinant protein production in Escherichia coli: Design of expression vectors and gene cloning
Cantoia, Alejo
; Aguilar Lucero, Dianela Ailin
; Ceccarelli, Eduardo Augusto
; Rosano, German Leandro
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Fecha de publicación:
08/2021
Editorial:
Elsevier Academic Press Inc.
Revista:
Methods In Enzymology
ISSN:
0076-6879
Idioma:
Inglés
Tipo de recurso:
Artículo publicado
Clasificación temática:
Resumen
Research in recombinant protein expression in microorganism hosts spans half a century. The field has evolved from mostly trial-and-error approaches to more rational strategies, including careful design of the expression vectors and the coding sequence for the protein of interest. It is important to reflect on many aspects about vector construction, such as codon usage, integration site, coding sequence mutagenesis and many others. In this chapter, we overview methods and considerations to generate a suitable construct and anticipate possible experimental roadblocks.
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Articulos(IBR)
Articulos de INST.DE BIOLOGIA MOLECULAR Y CELULAR DE ROSARIO
Articulos de INST.DE BIOLOGIA MOLECULAR Y CELULAR DE ROSARIO
Citación
Cantoia, Alejo; Aguilar Lucero, Dianela Ailin; Ceccarelli, Eduardo Augusto; Rosano, German Leandro; From the notebook to recombinant protein production in Escherichia coli: Design of expression vectors and gene cloning; Elsevier Academic Press Inc.; Methods In Enzymology; 659; 8-2021; 19-35
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