Artículo

Starting a new recombinant protein production project in Escherichia coli

Aguilar Lucero, Dianela AilinIcon ; Cantoia, AlejoIcon ; Ceccarelli, Eduardo AugustoIcon ; Rosano, German LeandroIcon
Fecha de publicación: 10/2021
Editorial: Elsevier Academic Press Inc.
Revista: Methods In Enzymology
ISSN: 0076-6879
Idioma: Inglés
Tipo de recurso: Artículo publicado
Clasificación temática:

Resumen

One of the goals in recombinant protein production in Escherichia coli is to maximize productivity. High volumetric and specific yields can be reached after careful selection of expression strains and optimization of cultivation parameters. In this chapter, we review the many tools available to make the most out of this versatile microbial cell factory. Useful guidelines and options for troubleshooting production are presented.
Palabras clave: CULTIVATION FACTORS , ESCHERICHIA COLI , INDUCER , PROTEIN AGGREGATION , RECOMBINANT PROTEINS
 
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Licencia
info:eu-repo/semantics/restrictedAccess Excepto donde se diga explícitamente, este item se publica bajo la siguiente descripción: Creative Commons Attribution-NonCommercial-ShareAlike 2.5 Unported (CC BY-NC-SA 2.5)
Identificadores
URI: http://hdl.handle.net/11336/182566
DOI: http://dx.doi.org/10.1016/bs.mie.2021.08.019
Colecciones
Articulos(IBR)
Articulos de INST.DE BIOLOGIA MOLECULAR Y CELULAR DE ROSARIO
Citación
Aguilar Lucero, Dianela Ailin; Cantoia, Alejo; Ceccarelli, Eduardo Augusto; Rosano, German Leandro; Starting a new recombinant protein production project in Escherichia coli; Elsevier Academic Press Inc.; Methods In Enzymology; 659; 10-2021; 3-18
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