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dc.contributor.author
Pan, Lei
dc.contributor.author
Gardner, Christopher L.
dc.contributor.author
Beliakoff, Reagan
dc.contributor.author
Da Silva, Danilo
dc.contributor.author
Zuo, Ran
dc.contributor.author
Pagliai, Fernando A.
dc.contributor.author
Padgett Pagliai, Kaylie A.
dc.contributor.author
Merli, Marcelo Luciano
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dc.contributor.author
Bahadiroglu, Erol
dc.contributor.author
Gonzalez, Claudio F.
dc.contributor.author
Lorca, Graciela L.
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dc.date.available
2022-12-27T15:37:59Z
dc.date.issued
2021-08
dc.identifier.citation
Pan, Lei; Gardner, Christopher L.; Beliakoff, Reagan; Da Silva, Danilo; Zuo, Ran; et al.; PrbP modulates biofilm formation in Liberibacter crescens; Wiley Blackwell Publishing, Inc; Environmental Microbiology; 23; 11; 8-2021; 7121-7138
dc.identifier.issn
1462-2912
dc.identifier.uri
http://hdl.handle.net/11336/182553
dc.description.abstract
In Liberibacter asiaticus, PrbP is a transcriptional regulatory protein involved in survival and persistence during host infection. Tolfenamic acid was previously found to inhibit interactions between PrbP and the promotor region of rplK, resulting in reduced survival of L. asiaticus in the citrus host. In this study, we performed transcriptome analyses to elucidate the PrbP regulon in L. crescens, as it is phylogenetically the closest related species to L. asiaticus that can be grown in laboratory conditions. Chemical inhibition of PrbP with tolfenamic acid revealed that PrbP is involved in the regulation of diverse cellular processes, including stress response, cell motility, cell cycle and biofilm formation. In vitro DNA binding and bacterial two-hybrid assays also suggested that PrbP is a global regulator of multiple transcription factors (RpoH, VisN, PleD, MucR, MocR and CtrA) at both transcriptional and/or post-transcriptional levels. Sub-lethal concentrations of tolfenamic acid significantly reduced the attachment of L. crescens during biofilm formation and decreased long-term persistence in biofilm structures. Overall, our findings show the importance of PrbP in regulating diverse biological processes through direct and indirect interactions with other transcriptional regulators in L. crescens.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Wiley Blackwell Publishing, Inc
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dc.rights
info:eu-repo/semantics/openAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
PrbP
dc.subject
transcriptional accessory protein
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Liberibacter crescens
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biofilm
dc.subject.classification
Bioquímica y Biología Molecular
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dc.subject.classification
Ciencias Biológicas
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dc.subject.classification
CIENCIAS NATURALES Y EXACTAS
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dc.title
PrbP modulates biofilm formation in Liberibacter crescens
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2022-09-19T16:03:15Z
dc.journal.volume
23
dc.journal.number
11
dc.journal.pagination
7121-7138
dc.journal.pais
Reino Unido
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dc.journal.ciudad
Londres
dc.description.fil
Fil: Pan, Lei. University of Florida. Department of Microbiology and Cell Science; Estados Unidos
dc.description.fil
Fil: Gardner, Christopher L.. University of Florida. Department of Microbiology and Cell Science; Estados Unidos
dc.description.fil
Fil: Beliakoff, Reagan. University of Florida. Department of Microbiology and Cell Science; Estados Unidos
dc.description.fil
Fil: Da Silva, Danilo. University of Florida. Department of Microbiology and Cell Science; Estados Unidos
dc.description.fil
Fil: Zuo, Ran. University of Florida. Department of Microbiology and Cell Science; Estados Unidos
dc.description.fil
Fil: Pagliai, Fernando A.. University of Florida. Department of Microbiology and Cell Science; Estados Unidos
dc.description.fil
Fil: Padgett Pagliai, Kaylie A.. University of Florida. Department of Microbiology and Cell Science; Estados Unidos
dc.description.fil
Fil: Merli, Marcelo Luciano. University of Florida. Department of Microbiology and Cell Science; Estados Unidos. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Biología Molecular y Celular de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario; Argentina
dc.description.fil
Fil: Bahadiroglu, Erol. University of Florida. Department of Microbiology and Cell Science; Estados Unidos
dc.description.fil
Fil: Gonzalez, Claudio F.. University of Florida. Department of Microbiology and Cell Science; Estados Unidos
dc.description.fil
Fil: Lorca, Graciela L.. University of Florida. Department of Microbiology and Cell Science; Estados Unidos
dc.journal.title
Environmental Microbiology
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dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://onlinelibrary.wiley.com/doi/10.1111/1462-2920.15740
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1111/1462-2920.15740
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