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dc.contributor.author
Echarren, María Laura
dc.contributor.author
Figueroa, Nicolás Raúl
dc.contributor.author
Vitor Horen, Luisina
dc.contributor.author
Pucciarelli, M. Graciela
dc.contributor.author
García el Portillo, Francisco
dc.contributor.author
Soncini, Fernando Carlos
dc.date.available
2022-12-27T14:41:21Z
dc.date.issued
2021-08
dc.identifier.citation
Echarren, María Laura; Figueroa, Nicolás Raúl; Vitor Horen, Luisina; Pucciarelli, M. Graciela; García el Portillo, Francisco; et al.; Balance between bacterial extracellular matrix production and intramacrophage proliferation by a Salmonella ‐specific SPI‐2‐encoded transcription factor; Wiley Blackwell Publishing, Inc; Molecular Microbiology; 116; 4; 8-2021; 1022-1032
dc.identifier.issn
0950-382X
dc.identifier.uri
http://hdl.handle.net/11336/182521
dc.description.abstract
Biosynthesis and secretion of a complex extracellular matrix (EM) is a hallmark of Salmonella biofilm formation, impacting on its relationship with both the environment and the host. Cellulose is a major component of Salmonella EM. It is considered an anti-virulence factor because it interferes with Salmonella proliferation inside macrophages and virulence in mice. Its synthesis is stimulated by CsgD, the master regulator of biofilm formation in enterobacteria, which in turn is under the control of MlrA, a MerR-like transcription factor. In this work, we identified a SPI-2-encoded Salmonella-specific transcription factor homolog to MlrA, MlrB, that represses transcription of its downstream gene, orf319, and of csgD inside host cells. MlrB is induced in laboratory media mimicking intracellular conditions and inside macrophages, and it is required for intramacrophage proliferation. An increased csgD expression is observed in the absence of MlrB inside host cells. Interestingly, inactivation of the CsgD-controlled cellulose synthase-coding gene restored intramacrophage proliferation to rates comparable to wild-type bacteria in the absence of MlrB. These data indicate that MlrB represses CsgD expression inside host cells and suggest that this repression lowers the activation of the cellulose synthase. Our findings provide a novel link between biofilm formation and Salmonella virulence.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Wiley Blackwell Publishing, Inc
dc.rights
info:eu-repo/semantics/restrictedAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
CELLULOSE-PRODUCTION
dc.subject
CSGD
dc.subject
MACROPHAGE SURVIVAL
dc.subject
MLRA-HOMOLOGUE
dc.subject.classification
Bioquímica y Biología Molecular
dc.subject.classification
Ciencias Biológicas
dc.subject.classification
CIENCIAS NATURALES Y EXACTAS
dc.title
Balance between bacterial extracellular matrix production and intramacrophage proliferation by a Salmonella ‐specific SPI‐2‐encoded transcription factor
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2022-09-07T17:22:59Z
dc.journal.volume
116
dc.journal.number
4
dc.journal.pagination
1022-1032
dc.journal.pais
Reino Unido
dc.journal.ciudad
Londres
dc.description.fil
Fil: Echarren, María Laura. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Biología Molecular y Celular de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario; Argentina
dc.description.fil
Fil: Figueroa, Nicolás Raúl. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Biología Molecular y Celular de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario; Argentina
dc.description.fil
Fil: Vitor Horen, Luisina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Biología Molecular y Celular de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario; Argentina
dc.description.fil
Fil: Pucciarelli, M. Graciela. Consejo Superior de Investigaciones Científicas; España. Universidad Autónoma de Madrid; España
dc.description.fil
Fil: García el Portillo, Francisco. Consejo Superior de Investigaciones Científicas; España
dc.description.fil
Fil: Soncini, Fernando Carlos. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Biología Molecular y Celular de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario; Argentina
dc.journal.title
Molecular Microbiology
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://onlinelibrary.wiley.com/doi/10.1111/mmi.14789
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1111/mmi.14789
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