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dc.contributor.author
Facchinetti, Maria Marta
dc.contributor.author
Gandini, Norberto Ariel
dc.contributor.author
Fermento, María Eugenia
dc.contributor.author
Sterin, Norma Beatriz
dc.contributor.author
Ji, Youngmi
dc.contributor.author
Patel, Vyomesh
dc.contributor.author
Gutkind, J. Silvio
dc.contributor.author
Rivadulla, Maria G.
dc.contributor.author
Curino, Alejandro Carlos
dc.date.available
2017-06-14T23:56:43Z
dc.date.issued
2010-10
dc.identifier.citation
Facchinetti, Maria Marta; Gandini, Norberto Ariel; Fermento, María Eugenia; Sterin, Norma Beatriz; Ji, Youngmi; et al.; The expression of Sphingosine kinase-1 in head and neck carcinoma; Karger; Cells Tissues Organs; 192; 5; 10-2010; 314-324
dc.identifier.issn
1422-6405
dc.identifier.uri
http://hdl.handle.net/11336/18232
dc.description.abstract
Sphingosine kinase-1 (SPHK1) modulates the proliferation, apoptosis and differentiation of keratinocytes through the regulation of ceramide and sphingosine-1-phosphate levels. However, studies on the expression of SPHK1 in human head and neck squamous cell carcinoma (HNSCC) specimens are lacking. Therefore, the aim of the present work was to evaluate SPHK1 expression in human primary HNSCCs and to correlate the results with clinical and anatomopathological parameters. We investigated the expression of this protein by immunohistochemistry performed in tissue microarrays of HNSCC and in an independent cohort of 37 paraffin-embedded specimens. SPHK1 expression was further validated by real-time PCR performed on laser capture-microdissected tissue samples. The positive rate of SPHK1 protein in the cancerous tissues was significantly higher (74%) than that in the nontumor oral tissues (23%), and malignant tissues showed stronger immunoreactivity for SPHK1 than normal matching samples. These results were confirmed by real-time PCR quantification of SPHK1 mRNA. Interestingly, the positive expression of SPHK1 was associated with shorter patient survival time (Kaplan-Meier survival curves) and with the loss of p21 expression. Taken together, these results demonstrate that SPHK1 is upregulated in HNSCC and provide clues of the role SPHK1 might play in tumor progression.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Karger
dc.rights
info:eu-repo/semantics/restrictedAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
Sphingosine Kinase
dc.subject
Oral Cancer
dc.subject
Sphingolipids
dc.subject
Tissue Microarray
dc.subject.classification
Bioquímica y Biología Molecular
dc.subject.classification
Ciencias Biológicas
dc.subject.classification
CIENCIAS NATURALES Y EXACTAS
dc.title
The expression of Sphingosine kinase-1 in head and neck carcinoma
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2017-06-14T14:35:24Z
dc.journal.volume
192
dc.journal.number
5
dc.journal.pagination
314-324
dc.journal.pais
Suiza
dc.journal.ciudad
Basilea
dc.description.fil
Fil: Facchinetti, Maria Marta. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina
dc.description.fil
Fil: Gandini, Norberto Ariel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina
dc.description.fil
Fil: Fermento, María Eugenia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina
dc.description.fil
Fil: Sterin, Norma Beatriz. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Biología Celular e Histología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
dc.description.fil
Fil: Ji, Youngmi. National Institutes of Health; Estados Unidos
dc.description.fil
Fil: Patel, Vyomesh. National Institutes of Health; Estados Unidos
dc.description.fil
Fil: Gutkind, J. Silvio. National Institutes of Health; Estados Unidos
dc.description.fil
Fil: Rivadulla, Maria G.. Hospital Municipal de Agudos "Dr. Leónidas Lucero"; Argentina
dc.description.fil
Fil: Curino, Alejandro Carlos. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina
dc.journal.title
Cells Tissues Organs
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1159/000318173
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://www.karger.com/Article/FullText/318173
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2974839/


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    Articulos de INST.DE QUIMICA Y FISICO-QUIMICA BIOLOGICAS "PROF. ALEJANDRO C. PALADINI"

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