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dc.contributor.author
Pérez García, Pablo
dc.contributor.author
Ma, Yuan
dc.contributor.author
Yanovsky, Marcelo Javier
dc.contributor.author
Mas, Paloma
dc.date.available
2017-06-14T21:12:21Z
dc.date.issued
2015-04
dc.identifier.citation
Pérez García, Pablo; Ma, Yuan; Yanovsky, Marcelo Javier; Mas, Paloma; Time-dependent sequestration of RVE8 by LNK proteins shapes the diurnal oscillation of anthocyanin biosynthesis; National Academy Of Sciences; Proceedings Of The National Academy Of Sciences Of The United States Of America; 112; 16; 4-2015; 5249-5253
dc.identifier.issn
0027-8424
dc.identifier.uri
http://hdl.handle.net/11336/18217
dc.description.abstract
Circadian clocks sustain 24-h rhythms in physiology and metabolism that are synchronized with the day/night cycle. In plants, the regulatory network responsible for the generation of rhythms has been broadly investigated over the past years. However, little is known about the intersecting pathways that link the environmental signals with rhythms in cellular metabolism. Here, we examine the role of the circadian components REVEILLE8/LHY-CCA1-LIKE5 (RVE8/LCL5) and NIGHT LIGHT-INDUCIBLE AND CLOCK-REGULATED genes (LNK) shaping the diurnal oscillation of the anthocyanin metabolic pathway. Around dawn, RVE8 up-regulates anthocyanin gene expression by directly associating to the promoters of a subset of anthocyanin biosynthetic genes. The up-regulation is overcome at midday by the repressing activity of LNK proteins, as inferred by the increased anthocyanin gene expression in lnk1/lnk2 double mutant plants. Chromatin immunoprecipitation assays using LNK and RVE8 misexpressing plants show that RVE8 binding to target promoters is precluded in LNK overexpressing plants and conversely, binding is enhanced in the absence of functional LNKs, which provides a mechanism by which LNKs antagonize RVE8 function in the regulation of anthocyanin accumulation. Based on their previously described transcriptional coactivating function, our study defines a switch in the regulatory activity of RVE8-LNK interaction, from a synergic coactivating role of evening-expressed clock genes to a repressive antagonistic function modulating anthocyanin biosynthesis around midday.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
National Academy Of Sciences
dc.rights
info:eu-repo/semantics/openAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
Anthocyanin Accumulation
dc.subject
Arabidopsis Thaliana
dc.subject
Circadian Clock
dc.subject
Protein-Protein Interaction
dc.subject
Transcriptional Regulation
dc.subject.classification
Bioquímica y Biología Molecular
dc.subject.classification
Ciencias Biológicas
dc.subject.classification
CIENCIAS NATURALES Y EXACTAS
dc.title
Time-dependent sequestration of RVE8 by LNK proteins shapes the diurnal oscillation of anthocyanin biosynthesis
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2017-06-12T21:00:02Z
dc.identifier.eissn
1091-6490
dc.journal.volume
112
dc.journal.number
16
dc.journal.pagination
5249-5253
dc.journal.pais
Estados Unidos
dc.description.fil
Fil: Pérez García, Pablo. Consejo Superior de Investigaciones Cientificas; España
dc.description.fil
Fil: Ma, Yuan. Consejo Superior de Investigaciones Cientificas; España
dc.description.fil
Fil: Yanovsky, Marcelo Javier. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Bioquímicas de Buenos Aires. Fundación Instituto Leloir. Instituto de Investigaciones Bioquímicas de Buenos Aires; Argentina
dc.description.fil
Fil: Mas, Paloma. Consejo Superior de Investigaciones Cientificas; España
dc.journal.title
Proceedings Of The National Academy Of Sciences Of The United States Of America
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/http://www.pnas.org/content/112/16/5249.long
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1073/pnas.1420792112
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