Artículo
An NMR‐based biosensor to measure stereo‐specific methionine sulfoxide reductase (MSR) activities in vitro and in vivo
Sanchez Lopez, Carolina; Labadie, Natalia
; Lombardo, Veronica Andrea
; Biglione, Franco Agustín
; Manta, Bruno; Jacob, Reeba; Gladyshev, Vadim; Abdelilah Seyfried, Salim; Selenko, Philipp; Binolfi, Andrés
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Fecha de publicación:
06/2020
Editorial:
Wiley VCH Verlag
Revista:
Chemistry- A European Journal
ISSN:
0947-6539
Idioma:
Inglés
Tipo de recurso:
Artículo publicado
Clasificación temática:
Resumen
Oxidation of protein methionines to methionine-sulfoxides (MetOx) is associated with several age-related diseases. In healthy cells, MetOx is reduced to methionine by two families of conserved methionine sulfoxide reductase enzymes, MSRA and MSRB that specifically target the S- or R-diastereoisomers of methionine-sulfoxides, respectively. To directly interrogate MSRA and MSRB functions in cellular settings, we developed an NMR-based biosensor that we call CarMetOx to simultaneously measure both enzyme activities in single reaction setups. We demonstrate the suitability of our strategy to delineate MSR functions in complex biological environments, including cell lysates and live zebrafish embryos. Thereby, we establish differences in substrate specificities between prokaryotic and eukaryotic MSRs and introduce CarMetOx as a highly sensitive tool for studying therapeutic targets of oxidative stress-related human diseases and redox regulated signaling pathways.
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Articulos(IBR)
Articulos de INST.DE BIOLOGIA MOLECULAR Y CELULAR DE ROSARIO
Articulos de INST.DE BIOLOGIA MOLECULAR Y CELULAR DE ROSARIO
Citación
Sanchez Lopez, Carolina; Labadie, Natalia; Lombardo, Veronica Andrea; Biglione, Franco Agustín; Manta, Bruno; et al.; An NMR‐based biosensor to measure stereo‐specific methionine sulfoxide reductase (MSR) activities in vitro and in vivo; Wiley VCH Verlag; Chemistry- A European Journal; 26; 65; 6-2020; 14838-14843
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