Repositorio Institucional
Repositorio Institucional
CONICET Digital
  • Inicio
  • EXPLORAR
    • AUTORES
    • DISCIPLINAS
    • COMUNIDADES
  • Estadísticas
  • Novedades
    • Noticias
    • Boletines
  • Ayuda
  • Acerca de
    • CONICET Digital
    • Equipo
    • Red Federal
JavaScript is disabled for your browser. Some features of this site may not work without it.
  • INFORMACIÓN GENERAL
  • RESUMEN
  • ESTADISTICAS
 
Artículo

Nitric oxide inhibits nitrate reductase activity in wheat leaves

Rosales, Eliana PaolaIcon ; Iannone, María FlorenciaIcon ; Groppa, María DanielaIcon ; Benavides, Maria PatriciaIcon
Fecha de publicación: 02/2011
Editorial: Elsevier Masson
Revista: Plant Physiology And Biochemistry
ISSN: 0981-9428
Idioma: Inglés
Tipo de recurso: Artículo publicado

Resumen

Nitrate reductase (NR), a committed enzyme in nitrate assimilation, is involved in the generation of nitric oxide (NO) in plants. In wheat leaf segments exposed to sodium nitroprusside (SNP) or S-nitrosoglutathione (GSNO), NR activity was significantly reduced to different degrees between 3 and 21 h, whereas its activity was partially recovered when the NO scavenger cPTIO was used. At 21 h, NR activity decreased from 38% with 10 μM SNP to 91% with 500 μM SNP, respect to the C values. S-nitrosoglutathione reduced NR activity between 18% and 26% only at 3 h. When added directly to the incubation solution, NR activity was quickly and strongly inhibited more than 90% by 10 or 50 μM SNP, whereas 10 μM GSNO reduced the enzyme activity an average of 50%, at 30 min of incubation. l-NAME and d-arginine (nitric oxide synthase (NOS) inhibitors) increased NR activity by 14% and 52% respectively, at 21 h of exposure, leading us to suppose that endogenous NOS-dependent NO formation could also be modulating NR activity. NR protein expression was not affected by 10 or 100 μM SNP at 3 or 21 h of incubation, whereas nitration of tyrosines was not detected in the NR protein. Nitrates, which content increased along the time in the tissues, could be exerting a role in this regulation.
Palabras clave: Nitrate Reductase , Nitric Oxide , Nitrogen Metabolism , Nitrates , Triticum Aestivum L
Ver el registro completo
 
Archivos asociados
Tamaño: 666.8Kb
Formato: PDF
.
Solicitar
Licencia
info:eu-repo/semantics/restrictedAccess Excepto donde se diga explícitamente, este item se publica bajo la siguiente descripción: Atribución-NoComercial-SinDerivadas 2.5 Argentina (CC BY-NC-ND 2.5 AR)
Identificadores
URI: http://hdl.handle.net/11336/18184
URL: http://www.sciencedirect.com/science/article/pii/S0981942810002366
DOI: http://dx.doi.org/10.1016/j.plaphy.2010.10.009
Colecciones
Articulos(IQUIFIB) [437]
Articulos de INST.DE QUIMICA Y FISICO-QUIMICA BIOLOGICAS "PROF. ALEJANDRO C. PALADINI"
Citación
Rosales, Eliana Paola; Iannone, María Florencia; Groppa, María Daniela; Benavides, Maria Patricia; Nitric oxide inhibits nitrate reductase activity in wheat leaves; Elsevier Masson; Plant Physiology And Biochemistry; 49; 2; 2-2011; 124-130
Compartir
Altmétricas
 
Estadísticas
Visualizaciones: 117
Descargas: 0

Enviar por e-mail
Separar cada destinatario (hasta 5) con punto y coma.
  • Facebook
  • Twitter
  • Instagram
  • YouTube
  • Sound Cloud

Los contenidos del CONICET están licenciados bajo Creative Commons Reconocimiento 2.5 Argentina License

Ministerio
https://www.conicet.gov.ar/ - CONICET

Inicio

Explorar

  • Autores
  • Disciplinas
  • Comunidades

Estadísticas

Novedades

  • Noticias
  • Boletines

Ayuda

Acerca de

  • CONICET Digital
  • Equipo
  • Red Federal
Godoy Cruz 2290 (C1425FQB) CABA – República Argentina – Tel: +5411 4899-5400 repositorio@conicet.gov.ar
TÉRMINOS Y CONDICIONES