Evento
Study of genotoxicity and citotoxicity of MccJ25 (G12Y)
Koltan, Mariano
; Bulacio Gil, Natalia María
; Rubio Molina, Anna Carolina
; Zenoff, Ana Maria
; Caram Di Santo, Maria Carolina
; de Cristobal, Ricardo Ezequiel
; Adler, Conrado
; Vincent, Paula Andrea
; Pomares, Maria Fernanda
Tipo del evento:
Reunión
Nombre del evento:
The LV Annual SAIB Meeting and XIV PABMB Congress
Fecha del evento:
05/11/2019
Institución Organizadora:
Sociedad Argentina de Investigación Bioquímica y Biología Molecular;
Panamerican Association of Biochemestry and Molecular Biology;
Título de la revista:
Biocell
Editorial:
Tech Science Press
ISSN:
1667-5746
Idioma:
Inglés
Clasificación temática:
Resumen
MccJ25 (G12Y) is an antimicrobial peptide active against E. coli O157 H: 7 and other diarrheagenic E. coli strains. MccJ25(G12Y) presents advantageous properties as a food preservative: it resists toextreme temperatures, it?s stable in a wide range of pH and exerts antimicrobial activity in food samples (egg yolk and milk). Our long-term aim is to develop a food preservative based on MccJ25 (G12Y).Therefore, we were interested in studying MccJ25 (G12Y) safety for humans. For this, we evaluated MccJ25 (G12Y)?s cytotoxicity on Artemia salina and Galleria mellonella, and its acute toxicity in BALB-cmice. In addition, we performed Ames test in order to test the peptide?s mutagenic potential. Results obtained confirmed the absence of genotoxic effects of MccJ25 (G12Y) on S. typhimurium.Furthermore, the assay performed in Artemia salina revealed that 0.31, 0.62, 1.25, 2.25 and 3 mg/ml of MccJ25 (G12Y) did not significantly affect their viability. In fact, the LD50 was not reached with anyof the MccJ25 (G12Y) concentrations tested. When MccJ25 (G12Y) citotoxicity was evaluated in Galleria mellonella, low toxicity was observed with the studied concentrations (1500 and 2500 mg/kg).Finally, acute toxicity was studied in female BALB-c mice. Neither death nor symptoms of toxicity in animals were observed. In addition, macroscopic pathological studies showed that the organs of MccJ25(G12Y)-treated animals did not present any morphological alteration. Taken together, results indicate that MccJ25 (G12Y) could be safe for humans. This finding is relevant in view of its application as anatural preservative to extend foods? shelf-life.
Palabras clave:
genotoxicity
,
citotoxicity
,
MccJ25 (G12Y)
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Eventos(INSIBIO)
Eventos de INST.SUP.DE INVEST.BIOLOGICAS
Eventos de INST.SUP.DE INVEST.BIOLOGICAS
Eventos(PROIMI)
Eventos de PLANTA PILOTO DE PROC.IND.MICROBIOLOGICOS (I)
Eventos de PLANTA PILOTO DE PROC.IND.MICROBIOLOGICOS (I)
Citación
Study of genotoxicity and citotoxicity of MccJ25 (G12Y); The LV Annual SAIB Meeting and XIV PABMB Congress; Salta; Argentina; 2019; 1-6
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