Artículo
Changes in digestive enzyme activities during the early ontogeny of the South American cichlid (Cichlasoma dimerus)
Toledo Solís, Francisco Javier; Hilerio Ruiz, Andrea Guadalupe; Delgadin, Tomás Horacio
; Pérez Sirkin, Daniela Irina
; Di Yorio, María Paula
; Vissio, Paula Gabriela
; Peña Marín, Emyr Saul; Martínez García, Rafael; Maytorena Verdugo, Claudia Ivette; Alvarez González, Carlos Alfonso; de Rodrigáñez, Miguel Ángel Sáenz
Fecha de publicación:
08/2021
Editorial:
Springer
Revista:
Fish Physiology and Biochemistry
ISSN:
0920-1742
Idioma:
Inglés
Tipo de recurso:
Artículo publicado
Clasificación temática:
Resumen
Cichlasoma dimerus is a neotropical cichlid that has been used as a biological model for neuroendocrinology studies. However, its culture is problematic in terms of larval feeding to allow having enough fry quantity and quality. Larviculture requires full knowledge about the digestive system and nutrition; therefore, this study was intended to assess the digestive enzymes' changes at different ages during the early ontogeny. Acid protease activity was detectable from the first day after hatching (dah), increasing to its maximum peaks on 9 dah. In contrast, alkaline proteases had low activity in the first days of life but reached their maximum activity on 17 dah. Chymotrypsin, L-aminopeptidase, and carboxypeptidase A activities increased at 6 dah, while trypsin activity was first detected on 13 dah and reached its maximum activity on 17 dah. Lipase and α-amylase activity were detectable at low levels in the first days of life, but the activity fluctuated and reaching its maximum activity at 21 dah. Alkaline phosphatase continued to oscillate and had two maximum activity peaks, the first at 6 dah and the second at 19 dah. Zymograms of alkaline proteases on day 6 dah six revealed four activity bands with molecular weights from 16.1 to 77.7 kDa. On 13 dah, two more activity bands of 24.4 and 121.9 kDa were detected, having a total of six proteases. The enzymatic activity analyzes indicate the digestive system shows the low activity of some enzymes in the first days after hatching, registering significant increases on 6 dah and the maximum peaks of activities around at 17 dah. Therefore, we recommend replacing live food with dry feed and only providing dry feed after day 17 dah.
Palabras clave:
CICHLASOMA DIMERUS
,
LIPASE
,
PROTEASES
,
ZYMOGRAMS
,
Α-AMYLASE
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Articulos(IBBEA)
Articulos de INSTITUTO DE BIODIVERSIDAD Y BIOLOGIA EXPERIMENTAL Y APLICADA
Articulos de INSTITUTO DE BIODIVERSIDAD Y BIOLOGIA EXPERIMENTAL Y APLICADA
Citación
Toledo Solís, Francisco Javier; Hilerio Ruiz, Andrea Guadalupe; Delgadin, Tomás Horacio; Pérez Sirkin, Daniela Irina; Di Yorio, María Paula; et al.; Changes in digestive enzyme activities during the early ontogeny of the South American cichlid (Cichlasoma dimerus); Springer; Fish Physiology and Biochemistry; 47; 4; 8-2021; 1211-1227
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