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Artículo

Antimicrobial peptidomes of Bothrops atrox and Bothrops jararacussu snake venoms

Alves Da Silva Caldeira, Cleopatra; Diniz Sousa, Rafaela; Carvalho Pimenta, Daniel; Azevedo dos Santos, Ana Paula; Bioni Garcia Teles, Carolina; Benevides Matos, Najla; da Silva, Saulo Luís; Guerino Stabeli, Rodrigo; Camperi, Silvia AndreaIcon ; Soares, Andreimar Martins; de Azevedo Calderon, Leonardo
Fecha de publicación: 07/2021
Editorial: Springer
Revista: Amino Acids
ISSN: 0939-4451
Idioma: Inglés
Tipo de recurso: Artículo publicado
Clasificación temática:
Otras Ciencias Químicas

Resumen

The worrisome emergence of pathogens resistant to conventional drugs has stimulated the search for new classes of antimicrobial and antiparasitic agents from natural sources. Antimicrobial peptides (AMPs), acting through mechanisms that do not rely on the interaction with a specific receptor, provide new possibilities for the development of drugs against resistant organisms. This study sought to purify and proteomically characterize the antimicrobial and antiparasitic peptidomes of B. atrox and B. jararacussu snake venoms against Gram-positive (Staphylococcus aureus, Methicillin-resistant Staphylococcus aureus—MRSA), Gram-negative (Escherichia coli, Pseudomonas aeruginosa, Klebsiella pneumoniae) bacteria, and the protozoan parasites Leishmania amazonensis and Plasmodium falciparum (clone W2, resistant to chloroquine). To this end, B. atrox and B. jararacussu venom peptides were purified by combination of 3 kDa cut-off Amicon® ultracentrifugal filters and reverse-phase high-performance liquid chromatography, and then identified by electrospray-ionization Ion-Trap/Time-of-Flight mass spectrometry. Fourteen distinct peptides, with masses ranging from 443.17 to 1383.73 Da and primary structure between 3 and 13 amino acid residues, were sequenced. Among them, 13 contained unique sequences, including 4 novel bradykinin-potentiating-like peptides (BPPs), and a snake venom metalloproteinase tripeptide inhibitor (SVMPi). Although commonly found in Viperidae venoms, except for Bax-12, the BPPs and SVMPi here reported had not been described in B. atrox and B. jararacussu venoms. Among the novel peptides, some exhibited bactericidal activity towards P. aeruginosa and S. aureus, had low hemolytic effect, and were devoid of antiparasitic activity. The identified novel antimicrobial peptides may be relevant in the development of new drugs for the management of multidrug-resistant Gram-negative and Gram-positive bacteria.
Palabras clave: ANTIMICROBIAL PEPTIDE , BOTHROPS ATROX , BOTHROPS JARARACUSSU , PEPTIDOMICS , SNAKE VENOM PEPTIDOME
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info:eu-repo/semantics/restrictedAccess Excepto donde se diga explícitamente, este item se publica bajo la siguiente descripción: Creative Commons Attribution-NonCommercial-ShareAlike 2.5 Unported (CC BY-NC-SA 2.5)
Identificadores
URI: http://hdl.handle.net/11336/181468
DOI: https://doi.org/10.1007/s00726-021-03055-y
URL: https://link.springer.com/article/10.1007/s00726-021-03055-y
Colecciones
Articulos(NANOBIOTEC)
Articulos de INSTITUTO DE NANOBIOTECNOLOGIA
Citación
Alves Da Silva Caldeira, Cleopatra; Diniz Sousa, Rafaela; Carvalho Pimenta, Daniel; Azevedo dos Santos, Ana Paula; Bioni Garcia Teles, Carolina; et al.; Antimicrobial peptidomes of Bothrops atrox and Bothrops jararacussu snake venoms; Springer; Amino Acids; 53; 10; 7-2021; 1635-1648
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