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dc.contributor.author
Peralta, Maria Patricia  
dc.contributor.author
Delgado, Osvaldo Daniel  
dc.contributor.author
Lechner, Bernardo Ernesto  
dc.contributor.author
Fariña, Julia Ines  
dc.date.available
2022-12-15T16:15:09Z  
dc.date.issued
2021  
dc.identifier.citation
Pptimization of ph for l-dopa production in bench-top scale stirredtank bioreactor using a Paraboeremia strain; LVI Annual Meeting Argentine Society for Biochemistry and Molecular Biology and XV Annual Meeting Argentinean Society for General Microbiology; Ciudad Autónoma de Buenos Aires; Argentina; 2020; 1-4  
dc.identifier.issn
0327-9545  
dc.identifier.uri
http://hdl.handle.net/11336/181349  
dc.description.abstract
The main success of a bioreactor is simulating environmental conditions which are favorable for microbial growth, as well as for the secretion of enzymes and the production of further metabolites. Therefore, the bioprocess development requires to optimize the conditions under which this bioreactor is operated. As the volume scale is raised, aeration and agitation conditions become more compromised, so their optimization is a must. The objective of this work was to study the influence of aeration and agitation conditions during L-Dopa production at bench-top bioreactor scale by using the Paraboeremia LY 38.7 fungal isolate selected from Las Yungas Tucumanas. First, operative conditions of aeration (1 vvm), temperature (25 °C) and pH (pHi = 7) were kept constant, whilst varying agitation parameter between 250, 200 and 150 rpm. Once the agitation parameter was optimized, different aeration values were evaluated (0.5, 1 and 1.5 vvm). Production of L-Dopa and consumption of L-Tyrosine were measured by means the Arnow method, tyrosinase monophenolase and diphenolase activities according to the dopachrome method, and biomass was estimated as dry weight. From the set of tested conditions, an agitation speed of 150 rpm and an airflow rate of 1 vvm allowed obtaining the highest L-Dopa production (229,04 mg?L-1) in 56 h. At the higher aeration value (1.5 vvm) L-Dopa production became decreased (162,69 mg?L-1), and the same happened at the higher agitation set point (107,94 mg?L-1), along with the production of a polymer. At lower agitation and aeration, less production of L-Dopa (94,57 mg?L-1 and 130,94 mg?L-1) was found as well as less fungal growth, so that the decrease in production could be due to a lower biosynthetic machinery which depends on biomass. Selected conditions would be advantageous from the operational point of view, particularly considering the subsequent bioprocess scale-up, since they would imply lower operating costs.  
dc.format
application/pdf  
dc.language.iso
eng  
dc.publisher
Tech Science Press  
dc.rights
info:eu-repo/semantics/openAccess  
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/  
dc.subject
PARABOEREMIA  
dc.subject
BIOREACTOR  
dc.subject
AERATION-AGITATION  
dc.subject
TIROSINASA-L-DOPA  
dc.subject.classification
Bioprocesamiento Tecnológico, Biocatálisis, Fermentación  
dc.subject.classification
Biotecnología Industrial  
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INGENIERÍAS Y TECNOLOGÍAS  
dc.title
Pptimization of ph for l-dopa production in bench-top scale stirredtank bioreactor using a Paraboeremia strain  
dc.type
info:eu-repo/semantics/publishedVersion  
dc.type
info:eu-repo/semantics/conferenceObject  
dc.type
info:ar-repo/semantics/documento de conferencia  
dc.date.updated
2022-12-12T23:15:29Z  
dc.journal.pagination
1-4  
dc.journal.pais
Argentina  
dc.journal.ciudad
Ciudad Autónoma de Buenos Aires  
dc.description.fil
Fil: Peralta, Maria Patricia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina  
dc.description.fil
Fil: Delgado, Osvaldo Daniel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina  
dc.description.fil
Fil: Lechner, Bernardo Ernesto. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Micología y Botánica. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Micología y Botánica; Argentina  
dc.description.fil
Fil: Fariña, Julia Ines. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina  
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/http://www.samige.org.ar/admin/news/files/170-TSP_BIOCELL_42376.pdf  
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Autor  
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Autor  
dc.conicet.rol
Autor  
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Autor  
dc.coverage
Internacional  
dc.type.subtype
Reunión  
dc.description.nombreEvento
LVI Annual Meeting Argentine Society for Biochemistry and Molecular Biology and XV Annual Meeting Argentinean Society for General Microbiology  
dc.date.evento
2020-11  
dc.description.ciudadEvento
Ciudad Autónoma de Buenos Aires  
dc.description.paisEvento
Argentina  
dc.type.publicacion
Journal  
dc.description.institucionOrganizadora
Sociedad Argentina de Investigación Bioquímica y Biología Molecular  
dc.description.institucionOrganizadora
Sociedad Argentina de Microbiología General  
dc.source.revista
Biocell  
dc.type
Reunión