Artículo
Cytotoxicity and DNA damage evaluation of TiO2 and ZnO nanoparticles. Uptake in lung cells in culture
Fecha de publicación:
03/2021
Editorial:
Oxford University Press
Revista:
Toxicology Research
e-ISSN:
2045-4538
Idioma:
Inglés
Tipo de recurso:
Artículo publicado
Clasificación temática:
Resumen
The cytotoxicity and DNA damage of titanium dioxide and zinc oxide nanoparticles (TiO2 and ZnO NPs) have been studied in a human lung carcinoma cell line (A549) after 24 h exposure. TiO2 and ZnO NPs had mean diameters of 12.9 ± 2.8 and 24.1 ± 8.0 nm, respectively. ZnO NPs reduced cell viability from 250 μg/mL, increasing reactive oxygen species (ROS) and decreased GSH/GSSG ratio. The comet assay detected DNA damage from 50 μg/mL. TiO2 NPs induced cytotoxicity and DNA damage from 50 to 100 μg/mL, respectively, along with a decrease of the GSH/GSSG ratio. Both particles were found inside the cells, within membrane-bound vesicles. The internalization mechanism is promoted partially by caveolae-mediated endocytosis and, in the case of TiO2 NPs, also by macropinocytosis.
Archivos asociados
Licencia
Identificadores
Colecciones
Articulos(CEQUINOR)
Articulos de CENTRO DE QUIMICA INORGANICA "DR. PEDRO J. AYMONINO"
Articulos de CENTRO DE QUIMICA INORGANICA "DR. PEDRO J. AYMONINO"
Citación
Freire, K.; Ordoñez Ramos, Fredy; Soria, Delia Beatriz; Pabón Gelves, E.; Di Virgilio, Ana Laura; Cytotoxicity and DNA damage evaluation of TiO2 and ZnO nanoparticles. Uptake in lung cells in culture; Oxford University Press; Toxicology Research; 10; 2; 3-2021; 264-271
Compartir
Altmétricas