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dc.contributor.author
Mukhamedova, Liya
dc.contributor.author
Füzik, Tibor
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Novácek, Jirí
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Hrebík, Dominik
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Pridal, Antonín
dc.contributor.author
Marti, Gerardo Anibal
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dc.contributor.author
Guérin, Diego M. A.
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Plevka, Pavel
dc.date.available
2022-12-14T13:12:01Z
dc.date.issued
2021-05
dc.identifier.citation
Mukhamedova, Liya; Füzik, Tibor; Novácek, Jirí; Hrebík, Dominik; Pridal, Antonín; et al.; Virion structure and in vitro genome release mechanism of dicistrovirus Kashmir Bee virus; American Society for Microbiology; Journal of Virology; 95; 11; 5-2021; 1-11
dc.identifier.issn
0022-538X
dc.identifier.uri
http://hdl.handle.net/11336/181092
dc.description.abstract
Infections with Kashmir bee virus (KBV) are lethal for honeybees and have been associated with colony collapse disorder. KBV and closely related viruses contribute to the ongoing decline in the number of honeybee colonies in North America, Europe, Australia, and other parts of the world. Despite the economic and ecological impact of KBV, its structure and infection process remain unknown. Here, we present the structure of the virion of KBV determined to a resolution of 2.8Å. We show that the exposure of KBV to acidic pH induces a reduction in interpentamer contacts within capsids and the reorganization of its RNA genome from a uniform distribution to regions of high and low density. Capsids of KBV crack into pieces at acidic pH, resulting in the formation of open particles lacking pentamers of capsid proteins. The large openings of capsids enable the rapid release of genomes and thus limit the probability of their degradation by RNases. The opening of capsids may be a shared mechanism for the genome release of viruses from the family Dicistroviridae. IMPORTANCE The western honeybee (Apis mellifera) is indispensable for maintaining agricultural productivity as well as the abundance and diversity of wild flowering plants. However, bees suffer from environmental pollution, parasites, and pathogens, including viruses. Outbreaks of virus infections cause the deaths of individual honeybees as well as collapses of whole colonies. Kashmir bee virus has been associated with colony collapse disorder in the United States, and no cure for the disease is currently available. Here, we report the structure of an infectious particle of Kashmir bee virus and show how its protein capsid opens to release the genome. Our structural characterization of the infection process determined that therapeutic compounds stabilizing contacts between pentamers of capsid proteins could prevent the genome release of the virus.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
American Society for Microbiology
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dc.rights
info:eu-repo/semantics/openAccess
dc.rights.uri
https://creativecommons.org/licenses/by/2.5/ar/
dc.subject
CAPSID OPENING
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GENOME RELEASE
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IFLAVIRUS
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RNA
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Virología
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Ciencias Biológicas
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dc.subject.classification
CIENCIAS NATURALES Y EXACTAS
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dc.title
Virion structure and in vitro genome release mechanism of dicistrovirus Kashmir Bee virus
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2022-09-21T23:35:56Z
dc.journal.volume
95
dc.journal.number
11
dc.journal.pagination
1-11
dc.journal.pais
Estados Unidos
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dc.journal.ciudad
Washington D.C
dc.description.fil
Fil: Mukhamedova, Liya. Masaryk University; República Checa
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Fil: Füzik, Tibor. Masaryk University; República Checa
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Fil: Novácek, Jirí. Masaryk University; República Checa
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Fil: Hrebík, Dominik. Masaryk University; República Checa
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Fil: Pridal, Antonín. Mendel University; República Checa
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Fil: Marti, Gerardo Anibal. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Estudios Parasitológicos y de Vectores. Universidad Nacional de La Plata. Facultad de Ciencias Naturales y Museo. Centro de Estudios Parasitológicos y de Vectores; Argentina
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Fil: Guérin, Diego M. A.. Universidad del País Vasco; España. Consejo Superior de Investigaciones Científicas; España. Instituto Biofisika; España
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Fil: Plevka, Pavel. Masaryk University; República Checa
dc.journal.title
Journal of Virology
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dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/http://jvi.asm.org/lookup/doi/10.1128/JVI.01950-20
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1128/JVI.01950-20
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