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dc.contributor.author
Wasinski, Frederick
dc.contributor.author
Barrile, Franco
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dc.contributor.author
Pedroso, João A. B.
dc.contributor.author
Quaresma, Paula G. F.
dc.contributor.author
Dos Santos, Willian O.
dc.contributor.author
List, Edward O.
dc.contributor.author
Kopchick, John J.
dc.contributor.author
Perelló, Mario
dc.contributor.author
Donato, Jose
dc.date.available
2022-12-14T11:23:35Z
dc.date.issued
2021-07
dc.identifier.citation
Wasinski, Frederick; Barrile, Franco; Pedroso, João A. B.; Quaresma, Paula G. F.; Dos Santos, Willian O.; et al.; Ghrelin-induced Food Intake, but not GH Secretion, Requires the Expression of the GH Receptor in the Brain of Male Mice; Endocrine Society; Endocrinology; 162; 7; 7-2021; 1-15
dc.identifier.issn
0013-7227
dc.identifier.uri
http://hdl.handle.net/11336/181066
dc.description.abstract
Ghrelin stimulates both GH secretion and food intake. The orexigenic action of ghrelin is mainly mediated by neurons that coexpress agouti-related protein (AgRP) and neuropeptide Y (NPY) in the arcuate nucleus of the hypothalamus (ARH). GH also stimulates food intake and, importantly, ARHAgRP/NPY neurons express GH receptor (GHR). Thus, ghrelin-induced GH secretion may contribute to the orexigenic effect of ghrelin. Here, we investigated the response to ghrelin in male mice carrying GHR ablation specifically in neurons (brain GHR knockout [KO] mice) or exclusively in ARHAgRP/NPY neurons (AgRP GHR KO mice). Although brain GHR KO mice showed normal ghrelin-induced increase in plasma GH levels, these mutants lacked the expected orexigenic response to ghrelin. Additionally, brain GHR KO mice displayed reduced hypothalamic levels of Npy and Ghsr mRNA and did not elicit ghrelin-induced c-Fos expression in the ARH. Furthermore, brain GHR KO mice exhibited a prominent reduction in AgRP fiber density in the ARH and paraventricular nucleus of the hypothalamus (PVH). In contrast, AgRP GHR KO mice showed no changes in the hypothalamic Npy and Ghsr mRNAs and conserved ghrelin-induced food intake and c-Fos expression in the ARH. AgRP GHR KO mice displayed a reduced AgRP fiber density (∼16%) in the PVH, but this reduction was less than that observed in brain GHR KO mice (∼61%). Our findings indicate that GHR signaling in the brain is required for the orexigenic effect of ghrelin, independently of GH action on ARHAgRP/NPY neurons.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Endocrine Society
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dc.rights
info:eu-repo/semantics/openAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
CYTOKINES
dc.subject
ENERGY BALANCE
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GROWTH HORMONE RECEPTOR
dc.subject
HYPOTHALAMUS
dc.subject.classification
Bioquímica y Biología Molecular
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dc.subject.classification
Ciencias Biológicas
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dc.subject.classification
CIENCIAS NATURALES Y EXACTAS
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dc.title
Ghrelin-induced Food Intake, but not GH Secretion, Requires the Expression of the GH Receptor in the Brain of Male Mice
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2022-09-21T16:53:47Z
dc.journal.volume
162
dc.journal.number
7
dc.journal.pagination
1-15
dc.journal.pais
Estados Unidos
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dc.description.fil
Fil: Wasinski, Frederick. Universidade de Sao Paulo; Brasil
dc.description.fil
Fil: Barrile, Franco. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto Multidisciplinario de Biología Celular. Provincia de Buenos Aires. Gobernación. Comisión de Investigaciones Científicas. Instituto Multidisciplinario de Biología Celular. Universidad Nacional de La Plata. Instituto Multidisciplinario de Biología Celular; Argentina
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Fil: Pedroso, João A. B.. Universidade de Sao Paulo; Brasil
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Fil: Quaresma, Paula G. F.. Universidade de Sao Paulo; Brasil
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Fil: Dos Santos, Willian O.. Universidade de Sao Paulo; Brasil
dc.description.fil
Fil: List, Edward O.. Ohio University; Estados Unidos
dc.description.fil
Fil: Kopchick, John J.. Ohio University; Estados Unidos
dc.description.fil
Fil: Perelló, Mario. Multidisciplinary Institute Of Cell Biology; Argentina
dc.description.fil
Fil: Donato, Jose. Universidade de Sao Paulo; Brasil
dc.journal.title
Endocrinology
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1210/endocr/bqab097
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