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dc.contributor.author
Han, Yong K.  
dc.contributor.author
Segall, Adriana Ines  
dc.date.available
2015-08-25T14:55:45Z  
dc.date.issued
2013-11  
dc.identifier.citation
Han, Yong K.; Segall, Adriana Ines; Validation of a Stability indicating RP-HPLC Method for the Determination of Gatifloxacin in eye drops; Research Trends; Trends in Chromatography; 8; 11-2013; 23-29  
dc.identifier.issn
0972-8635  
dc.identifier.uri
http://hdl.handle.net/11336/1809  
dc.description.abstract
A reversed-phase liquid chromatography (RP-LC) method was validated for the determination of gatifloxacin in eye drops. The LC method was carried out on a Phenomenex LiChrosphere 5 µm RP-18 100 Ǻ 125 x 4.6 mm maintained at room temperature. The mobile phase consisted of water:acetonitrile:triethylamine (80:20:3 v/v/v), pH adjusted to 3.3 using phosphoric acid, run at a flow rate of 1 mL/min and using ultraviolet detection at 293 nm. The chromatographic separation was obtained with a retention time of 3.6 min and was linear in the range of 30-70 µg/mL (r2 = 0.9991). The specificity and stability indicating the capability of the method was proven through forced degradation studies, which also showed that there was no interference of the excipients. The accuracy was 100.63% with RSD = 1.65. Method validation demonstrates satisfactory results for precision and robustness. The proposed method was applied for the analysis of marketed eye drops, for improving the quality control and to assure the therapeutic efficacy.  
dc.format
application/pdf  
dc.language.iso
eng  
dc.publisher
Research Trends  
dc.rights
info:eu-repo/semantics/openAccess  
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/  
dc.subject
Pridinol Mesylate  
dc.subject
Hplc  
dc.subject
Raw Material  
dc.subject
Gatifloxacin  
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Assay  
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Stability-Indicating Method  
dc.subject.classification
Química Analítica  
dc.subject.classification
Ciencias Químicas  
dc.subject.classification
CIENCIAS NATURALES Y EXACTAS  
dc.title
Validation of a Stability indicating RP-HPLC Method for the Determination of Gatifloxacin in eye drops  
dc.type
info:eu-repo/semantics/article  
dc.type
info:ar-repo/semantics/artículo  
dc.type
info:eu-repo/semantics/publishedVersion  
dc.date.updated
2016-03-30 10:35:44.97925-03  
dc.journal.volume
8  
dc.journal.pagination
23-29  
dc.journal.pais
India  
dc.journal.ciudad
Poojapura  
dc.description.fil
Fil: Han, Yong K.. Universidad de Buenos Aires. Facultad de Farmacia y Bioquimica. Departamento de Tecnologia Farmaceutica; Argentina;  
dc.description.fil
Fil: Segall, Adriana Ines. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Tecnología Farmacéutica; Argentina;  
dc.journal.title
Trends in Chromatography  
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/http://www.researchtrends.net/tia/abstract.asp?in=0&vn=8&tid=60&aid=4792&pub=2013&type=3