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Artículo

Molecular and physiological analysis of indole-3-acetic acid degradation in Bradyrhizobium japonicum E109

Torres, Daniela SoledadIcon ; Mongiardini, Elias JavierIcon ; Donadío, Evelyn FlorenciaIcon ; Donoso, Raúl; Recabarren Gajardo, Gonzalo; Gualpa, José LuisIcon ; Spaepen, Stijn; Defez, Roberto; Lopez, Gaston AlbertoIcon ; Bianco, Carmen; Cassan, Fabricio DarioIcon
Fecha de publicación: 02/2021
Editorial: Elsevier Science
Revista: Research In Microbiology
ISSN: 0923-2508
Idioma: Inglés
Tipo de recurso: Artículo publicado
Clasificación temática:
Biología Celular, Microbiología

Resumen

Bradyrhizobium japonicum E109 is a bacterium widely used for inoculants production in Argentina. It is known for its ability to produce several phytohormones and degrade indole-3-acetic acid (IAA). The genome sequence of B. japonicum E109 was recently analyzed and it showed the presence of genes related to the synthesis of IAA by indole-3-acetonitrile, indole-3-acetamide and tryptamine pathways. Nevertheless, B. japonicum E109 is not able to produce IAA and instead has the ability to degrade this hormone under saprophytic culture conditions. This work aimed to study the molecular and physiological features of IAA degradation and identify the genes responsible of this activity. In B. japonicum E109 we identified two sequences coding for a putative 3-phenylpropionate dioxygenase (subunits α and β) responsible for the IAA degradation that were homologous to the canonical cluster of iacC and iacD of Pseudomonas putida 1290. These genes form a separate cluster together with three additional genes with unknown functions. The degradation activity was found to be constitutively expressed in B. japonicum E109. As products of IAA degradation, we identified two compounds, 3-indoleacetic acid 2,3-oxide and 2-(2-hydroperoxy-3-hydroxyindolin-3-yl) acetic acid. Our report proposes, for the first time, a model for IAA degradation in Bradyrhizobium.
Palabras clave: 2-(2-HYDROPEROXY-3-HYDROXYINDOLIN-3-YL) ACETIC ACID , 3-INDOLEACETIC ACID 2 , 3-OXIDE , 3-PHENYLPROPIONATE DIOXYGENASE , B. JAPONICUM , INDOLE-3-ACETIC ACID
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info:eu-repo/semantics/openAccess Excepto donde se diga explícitamente, este item se publica bajo la siguiente descripción: Atribución-NoComercial-SinDerivadas 2.5 Argentina (CC BY-NC-ND 2.5 AR)
Identificadores
URI: http://hdl.handle.net/11336/180980
URL: https://linkinghub.elsevier.com/retrieve/pii/S0923250821000279
DOI: http://dx.doi.org/10.1016/j.resmic.2021.103814
Colecciones
Articulos(IBBM)
Articulos de INST.DE BIOTECNOLOGIA Y BIOLOGIA MOLECULAR
Citación
Torres, Daniela Soledad; Mongiardini, Elias Javier; Donadío, Evelyn Florencia; Donoso, Raúl; Recabarren Gajardo, Gonzalo; et al.; Molecular and physiological analysis of indole-3-acetic acid degradation in Bradyrhizobium japonicum E109; Elsevier Science; Research In Microbiology; 172; 3; 2-2021; 1-47
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