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dc.contributor.author
Herrera, Macarena Lorena  
dc.contributor.author
Bandín, Sandra  
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Champarini, Leandro Gabriel  
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Hereñú, Claudia Beatriz  
dc.contributor.author
Bellini, Maria Jose  
dc.date.available
2022-12-13T14:36:12Z  
dc.date.issued
2021-10  
dc.identifier.citation
Herrera, Macarena Lorena; Bandín, Sandra; Champarini, Leandro Gabriel; Hereñú, Claudia Beatriz; Bellini, Maria Jose; Intramuscular insulin-like growth factor-1 gene therapy modulates reactive microglia after traumatic brain injury; Pergamon-Elsevier Science Ltd; Brain Research Bulletin; 175; 10-2021; 196-204  
dc.identifier.issn
0361-9230  
dc.identifier.uri
http://hdl.handle.net/11336/180974  
dc.description.abstract
Reactive gliosis is a key feature and an important pathophysiological mechanism underlying chronic neurodegeneration following traumatic brain injury (TBI). In this study, we have explored the effects of intramuscular IGF-1 gene therapy on reactive gliosis and functional outcome after an injury of the cerebral cortex. Young adult male rats were intramuscularly injected with a recombinant adenoviral construct harboring the cDNA of human IGF-1 (RAd-IGF1), with a control vector expressing green fluorescent protein (RAd-GFP) or PBS as control. Three weeks after the intramuscular injections of adenoviral vectors, animals were subjected to a unilateral penetrating brain injury. The data revealed that RAd-IGF1 gene therapy significantly increased serum IGF1 levels and improved working memory performance after one week of TBI as compared to PBS or RAd-GFP lesioned animals. At the same time, when we analyzed the effects of therapy on glial scar formation, the treatment with RAd-IGF1 did not modify the number of glial fibrillary acidic protein (GFAP) positive cells, but we observed a decrease in vimentin immunoreactive astrocytes at 7 days post-lesion in the injured hemisphere compared to RAd-GFP group. Moreover, IGF-1 gene therapy reduced the number of Iba1+ cells with reactive phenotype and the number of MHCII + cells in the injured hemisphere. These results suggest that intramuscular IGF-1 gene therapy may represent a new approach to prevent traumatic brain injury outcomes in rats.  
dc.format
application/pdf  
dc.language.iso
eng  
dc.publisher
Pergamon-Elsevier Science Ltd  
dc.rights
info:eu-repo/semantics/restrictedAccess  
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/  
dc.subject
ASTROCYTES  
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IGF-1  
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MICROGLIA  
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NEUROINFLAMMATION  
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NEUROPROTECTION  
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TRAUMATIC BRAIN INJURY  
dc.subject.classification
Neurociencias  
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Medicina Básica  
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CIENCIAS MÉDICAS Y DE LA SALUD  
dc.title
Intramuscular insulin-like growth factor-1 gene therapy modulates reactive microglia after traumatic brain injury  
dc.type
info:eu-repo/semantics/article  
dc.type
info:ar-repo/semantics/artículo  
dc.type
info:eu-repo/semantics/publishedVersion  
dc.date.updated
2022-09-20T18:43:50Z  
dc.journal.volume
175  
dc.journal.pagination
196-204  
dc.journal.pais
Estados Unidos  
dc.description.fil
Fil: Herrera, Macarena Lorena. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Farmacología Experimental de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Instituto de Farmacología Experimental de Córdoba; Argentina  
dc.description.fil
Fil: Bandín, Sandra. Consejo Superior de Investigaciones Científicas; España  
dc.description.fil
Fil: Champarini, Leandro Gabriel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Farmacología Experimental de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Instituto de Farmacología Experimental de Córdoba; Argentina  
dc.description.fil
Fil: Hereñú, Claudia Beatriz. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Farmacología Experimental de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Instituto de Farmacología Experimental de Córdoba; Argentina  
dc.description.fil
Fil: Bellini, Maria Jose. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Bioquímicas de La Plata "Prof. Dr. Rodolfo R. Brenner". Universidad Nacional de la Plata. Facultad de Ciencias Médicas. Instituto de Investigaciones Bioquímicas de La Plata "Prof. Dr. Rodolfo R. Brenner"; Argentina  
dc.journal.title
Brain Research Bulletin  
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1016/j.brainresbull.2021.07.023  
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/abs/pii/S0361923021002331