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dc.contributor.author
Castro, Camila Andrea
dc.contributor.author
Zhang, Ruiyong
dc.contributor.author
Liu, Jing
dc.contributor.author
Bellenberg, Sören
dc.contributor.author
Neu, Thomas R.
dc.contributor.author
Donati, Edgardo Ruben
dc.contributor.author
Sand, Wolfgang
dc.contributor.author
Vera, Mario
dc.date.available
2022-12-12T11:58:30Z
dc.date.issued
2016-08
dc.identifier.citation
Castro, Camila Andrea; Zhang, Ruiyong; Liu, Jing; Bellenberg, Sören; Neu, Thomas R.; et al.; Biofilm Formation and Interspecies Interactions in Mixed Cultures of Thermoacidophilic Archaea Acidianus spp. and Sulfolobus metallicus; Elsevier Science; Research In Microbiology; 167; 7; 8-2016; 604-612
dc.identifier.issn
0923-2508
dc.identifier.uri
http://hdl.handle.net/11336/180691
dc.description.abstract
The understanding of biofilm formation by bioleaching microorganisms is of great importance for influencing mineral dissolution rates and to prevent acid mine drainage (AMD). Thermo-acidophilic archaea such as Acidianus, Sulfolobus and Metallosphaera are of special interest due to their ability to perform leaching at high temperatures, thereby enhancing leaching rates. In this work, leaching experiments and visualization by microscopy of cell attachment and biofilm formation patterns of the crenarchaeotes Sulfolobus metallicus DSM 6482T and the Acidianus isolates DSM 29038 and DSM 29099 in pure and mixed cultures on sulfur or pyrite were studied. Confocal laser scanning microscopy (CLSM) combined with fluorescent dyes as well as fluorescently labeled lectins were used to visualize different components (e.g. DNA, proteins or glycoconjugates) of the aforementioned species. The data indicate that cell attachment and the subsequently formed biofilms were species- and substrate-dependent. Pyrite leaching experiments coupled with pre-colonization and further inoculation with a second species suggest that both species may negatively influence each other during pyrite leaching with respect to initial attachment and pyrite dissolution rates. In addition, the investigation of binary biofilms on pyrite showed that both species were heterogeneously distributed on pyrite surfaces in the form of individual cells or microcolonies. Physical contact between the two species seems to occur, as revealed by specific lectins able to specifically bind single species within mixed cultures.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Elsevier Science
dc.rights
info:eu-repo/semantics/openAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
ACIDIANUS SPP
dc.subject
BIOFILM FORMATION
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INTERSPECIES INTERACTIONS
dc.subject
SULFOLOBUS METALLICUS
dc.subject
THERMO-ACIDOPHILIC ARCHAEA
dc.subject.classification
Otras Biotecnología Industrial
dc.subject.classification
Biotecnología Industrial
dc.subject.classification
INGENIERÍAS Y TECNOLOGÍAS
dc.title
Biofilm Formation and Interspecies Interactions in Mixed Cultures of Thermoacidophilic Archaea Acidianus spp. and Sulfolobus metallicus
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2022-12-07T18:00:13Z
dc.journal.volume
167
dc.journal.number
7
dc.journal.pagination
604-612
dc.journal.pais
Países Bajos
dc.journal.ciudad
Amsterdam
dc.description.fil
Fil: Castro, Camila Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Investigación y Desarrollo en Fermentaciones Industriales. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Centro de Investigación y Desarrollo en Fermentaciones Industriales; Argentina
dc.description.fil
Fil: Zhang, Ruiyong. Universitat Duisburg - Essen. Aquatische Biotechnologie Biofilm Centre; Alemania
dc.description.fil
Fil: Liu, Jing. Universitat Duisburg - Essen. Aquatische Biotechnologie Biofilm Centre; Alemania
dc.description.fil
Fil: Bellenberg, Sören. Universitat Duisburg - Essen. Aquatische Biotechnologie Biofilm Centre; Alemania
dc.description.fil
Fil: Neu, Thomas R.. Helmholtz Centre For Environmental Research-ufz; Alemania
dc.description.fil
Fil: Donati, Edgardo Ruben. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Investigación y Desarrollo en Fermentaciones Industriales. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Centro de Investigación y Desarrollo en Fermentaciones Industriales; Argentina
dc.description.fil
Fil: Sand, Wolfgang. Universitat Duisburg - Essen. Aquatische Biotechnologie Biofilm Centre; Alemania
dc.description.fil
Fil: Vera, Mario. Universitat Duisburg - Essen. Aquatische Biotechnologie Biofilm Centre; Alemania
dc.journal.title
Research In Microbiology
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S0923250816300675
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1016/j.resmic.2016.06.005
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