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Artículo

Expression profiling of echinococcus multilocularis mirnas throughout metacestode development in vitro

Macchiaroli, NataliaIcon ; Preza, Matías; Pérez, Matías GastónIcon ; Kamenetzky, LauraIcon ; Cucher, Marcela AlejandraIcon ; Koziol, Uriel; Castillo, Susana Estela; Berriman, Matthew; Brehm, Klaus; Rosenzvit, Mara CeciliaIcon
Fecha de publicación: 03/2021
Editorial: Public Library of Science
Revista: Neglected Tropical Diseases
ISSN: 1935-2735
Idioma: Inglés
Tipo de recurso: Artículo publicado
Clasificación temática:
Parasitología

Resumen

The neglected zoonotic disease alveolar echinococcosis (AE) is caused by the metacestode stage of the tapeworm parasite Echinococcus multilocularis. MicroRNAs (miRNAs) are small non-coding RNAs with a major role in regulating gene expression in key biological pro-cesses. We analyzed the expression profile of E. multilocularis miRNAs throughout meta-cestode development in vitro, determined the spatial expression of miR-71 in metacestodes cultured in vitro and predicted miRNA targets. Small cDNA libraries from different samples of E. multilocularis were sequenced. We confirmed the expression of 37 miRNAs in E. multi-locularis being some of them absent in the host, such as miR-71. We found a few miRNAs highly expressed in all life cycle stages and conditions analyzed, whereas most miRNAs showed very low expression. The most expressed miRNAs were miR-71, miR-9, let-7, miR-10, miR-4989 and miR-1. The high expression of these miRNAs was conserved in other tapeworms, suggesting essential roles in development, survival, or host-parasite interaction. We found highly regulated miRNAs during the different transitions or cultured conditions analyzed, which might suggest a role in the regulation of developmental timing, host-para-site interaction, and/or in maintaining the unique developmental features of each developmental stage or condition. We determined that miR-71 is expressed in germinative cells and in other cell types of the germinal layer in E. multilocularis metacestodes cultured in vitro. MiRNA target prediction of the most highly expressed miRNAs and in silico functional analysis suggested conserved and essential roles for these miRNAs in parasite biology. We found relevant targets potentially involved in development, cell growth and death, lifespan regulation, transcription, signal transduction and cell motility. The evolutionary conservation and expression analyses of E. multilocularis miRNAs throughout metacestode development along with the in silico functional analyses of their predicted targets might help to identify selective therapeutic targets for treatment and control of AE.
Palabras clave: Echinococcus multilocularis , Tapeworm , Cestoda , microRNA , metacestode , development , host-parasite interaction , microRNA in situ hybridization , miRNA targets
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info:eu-repo/semantics/openAccess Excepto donde se diga explícitamente, este item se publica bajo la siguiente descripción: Creative Commons Attribution-NonCommercial-ShareAlike 2.5 Unported (CC BY-NC-SA 2.5)
Identificadores
URI: http://hdl.handle.net/11336/180142
URL: https://journals.plos.org/plosntds/article?id=10.1371/journal.pntd.0009297
DOI: http://dx.doi.org/10.1371/journal.pntd.0009297
Colecciones
Articulos(IMPAM)
Articulos de INSTITUTO DE INVESTIGACIONES EN MICROBIOLOGIA Y PARASITOLOGIA MEDICA
Citación
Macchiaroli, Natalia; Preza, Matías; Pérez, Matías Gastón; Kamenetzky, Laura; Cucher, Marcela Alejandra; et al.; Expression profiling of echinococcus multilocularis mirnas throughout metacestode development in vitro; Public Library of Science; Neglected Tropical Diseases; 15; 3; 3-2021; 1-32
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