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dc.contributor.author
Kilstein, Yael  
dc.contributor.author
Nowak, Wanda  
dc.contributor.author
Errasti, Andrea Emilse  
dc.contributor.author
Feás, Antía Andrea Barcia  
dc.contributor.author
Armesto, Arnaldo Raúl  
dc.contributor.author
Pelorosso, Facundo German  
dc.contributor.author
Rothlin, Rodolfo Pedro  
dc.date.available
2022-12-01T17:38:29Z  
dc.date.issued
2016-04  
dc.identifier.citation
Kilstein, Yael; Nowak, Wanda; Errasti, Andrea Emilse; Feás, Antía Andrea Barcia; Armesto, Arnaldo Raúl; et al.; Involvement of extracellular signal-regulated kinase 5 in kinin B1 receptor upregulation in isolated human umbilical veins; American Society for Pharmacology and Experimental Therapeutics; Journal of Pharmacology and Experimental Therapeutics; 357; 1; 4-2016; 114-124  
dc.identifier.issn
0022-3565  
dc.identifier.uri
http://hdl.handle.net/11336/179859  
dc.description.abstract
The upregulated kinin B1 receptors exert a pivotal role in modulating inflammatory processes. In isolated human umbilical veins (HUVs), kinin B1 receptor is upregulated as a function of in vitro incubation time and proinflammatory stimuli. The aim of this study was to evaluate, using functional and biochemical methods, the involvement of extracellular signal-regulated kinase 5 (ERK5), p38 mitogen-activated protein kinase (MAPK), c-Jun N-terminal kinase (JNK), and extracellular signal-regulated kinase 1/2 (ERK1/ 2) on the kinin B1 receptor upregulation process in HUV. Real-time polymerase chain reaction analysis revealed for the first time that kinin B1 receptor mRNA expression closely parallels the functional sensitization to kinin B1 receptor selective agonist des-Arg10- kallidin (DAKD) in HUV. Moreover, the selective inhibition of ERK5, p38 MAPK, and JNK, but not ERK1/2, produced a dosedependent rightward shift of the concentration-response curves to DAKD after 5-hour incubation and a reduction in kinin B1 receptor mRNA expression. Biochemical analyses showed that ERK5, p38 MAPK, and JNK phosphorylation is maximal during the first 2 hours postisolation, followed by a significant reduction in the last 3 hours. None of the treatments modified the responses to serotonin, an unrelated agonist, suggesting a specific effect on kinin B1 receptor upregulation. The present work provides for the first time pharmacologic evidence indicating that ERK5 plays a significant role on kinin B1 receptor upregulation. Furthermore, we confirm the relevance of p38 MAPK and JNK as well as the lack of effect of ERK1/2 in this process. This study may contribute to a better understanding of MAPK involvement in inflammatory and immunologic diseases.  
dc.format
application/pdf  
dc.language.iso
eng  
dc.publisher
American Society for Pharmacology and Experimental Therapeutics  
dc.rights
info:eu-repo/semantics/openAccess  
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/  
dc.subject
AP-1  
dc.subject
ANTI-INFLAMMATORY DRUGS  
dc.subject
BRADYKININ RECEPTORS  
dc.subject
KINASES  
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RECEPTOR-UP REGULATION  
dc.subject.classification
Otras Ciencias de la Salud  
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Ciencias de la Salud  
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CIENCIAS MÉDICAS Y DE LA SALUD  
dc.title
Involvement of extracellular signal-regulated kinase 5 in kinin B1 receptor upregulation in isolated human umbilical veins  
dc.type
info:eu-repo/semantics/article  
dc.type
info:ar-repo/semantics/artículo  
dc.type
info:eu-repo/semantics/publishedVersion  
dc.date.updated
2022-12-01T11:25:22Z  
dc.journal.volume
357  
dc.journal.number
1  
dc.journal.pagination
114-124  
dc.journal.pais
Estados Unidos  
dc.journal.ciudad
Baltimore  
dc.description.fil
Fil: Kilstein, Yael. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Farmacología; Argentina  
dc.description.fil
Fil: Nowak, Wanda. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Farmacología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina  
dc.description.fil
Fil: Errasti, Andrea Emilse. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Farmacología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina  
dc.description.fil
Fil: Feás, Antía Andrea Barcia. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Farmacología; Argentina  
dc.description.fil
Fil: Armesto, Arnaldo Raúl. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Farmacología; Argentina  
dc.description.fil
Fil: Pelorosso, Facundo German. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Farmacología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina  
dc.description.fil
Fil: Rothlin, Rodolfo Pedro. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Farmacología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina  
dc.journal.title
Journal of Pharmacology and Experimental Therapeutics  
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://jpet.aspetjournals.org/content/357/1/114  
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1124/jpet.115.230169