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dc.contributor.author
Heredia, Romina Marisa
dc.contributor.author
Boeris, Paola Sabrina
dc.contributor.author
Liffourrena, Andres Sebastian
dc.contributor.author
Bergero, Maria Fernanda
dc.contributor.author
López, Gastón Alberto
dc.contributor.author
Lucchesi, Gloria Ines
dc.date.available
2022-11-30T19:07:16Z
dc.date.issued
2016-03
dc.identifier.citation
Heredia, Romina Marisa; Boeris, Paola Sabrina; Liffourrena, Andres Sebastian; Bergero, Maria Fernanda; López, Gastón Alberto; et al.; Release of outer membrane vesicles in pseudomonas putida as a response to stress caused by cationic surfactants; Society for General Microbiology; Microbiology-UK; 162; 5; 3-2016; 813-822
dc.identifier.issn
1350-0872
dc.identifier.uri
http://hdl.handle.net/11336/179637
dc.description.abstract
Pseudomonas putida A (ATCC 12633), a degrader of cationic surfactants, releases outer membrane vesicles (OMVs) when grown with tetradecyltrimethylammonium bromide (TTAB) as the sole carbon, nitrogen and energy source. The OMVs exhibit a bilayer structure and were found to be composed of lipopolysaccharides, proteins and phospholipids (PLs) such as cardiolipin, phosphatidylcholine, phosphatidic acid and phosphatidylglycerol (PG). The OMVs showed a marked increase in the PG content, approximately 43 % higher than the amount registered in the parent cells from which the vesicles were derived. After growth of P. putida with TTAB, the amount of lipoprotein covalently cross-linked to the peptidoglycan showed a twofold decrease when compared with values found after growth without the surfactant [16±2 and 28±3 mg (mg cell envelope protein)-1, respectively]. This decrease in the amount of lipoprotein can be related to areas of loss of contact between the outer membrane and the peptidoglycan and, therefore, to OMV production. In addition, due to its amphiphilic nature, TTAB can contribute to OMV biogenesis, through a physical mechanism, by induction of the curvature of the membrane. Taking into account that OVMs were produced when the cells were grown under external stress, caused by the surfactant, and that TTAB was detected in the vesicles [48 nmol TTAB (nmol PL)-1], we concluded that this system of TTAB elimination is a mechanism that P. putida A (ATCC 12633) would utilize for alleviating stress caused by cationic surfactants.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Society for General Microbiology
dc.rights
info:eu-repo/semantics/openAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
TETRADECYLTRIMETHYLAMMONIUM
dc.subject
PSEUDOMONAS PUTIDA
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VESICLES
dc.subject
TETRADECYLTRIMETHYLAMMONIUM
dc.subject.classification
Biología Celular, Microbiología
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Ciencias Biológicas
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CIENCIAS NATURALES Y EXACTAS
dc.title
Release of outer membrane vesicles in pseudomonas putida as a response to stress caused by cationic surfactants
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2022-11-30T12:05:43Z
dc.journal.volume
162
dc.journal.number
5
dc.journal.pagination
813-822
dc.journal.pais
Reino Unido
dc.journal.ciudad
London
dc.description.fil
Fil: Heredia, Romina Marisa. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Biología Molecular; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; Argentina
dc.description.fil
Fil: Boeris, Paola Sabrina. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Biología Molecular; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; Argentina
dc.description.fil
Fil: Liffourrena, Andres Sebastian. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Biología Molecular; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; Argentina
dc.description.fil
Fil: Bergero, Maria Fernanda. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Biología Molecular; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; Argentina
dc.description.fil
Fil: López, Gastón Alberto. Universidad Nacional de Río Cuarto; Argentina
dc.description.fil
Fil: Lucchesi, Gloria Ines. Universidad Nacional de Río Cuarto; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; Argentina
dc.journal.title
Microbiology-UK
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/https://doi.org/10.1099/mic.0.000265
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://www.microbiologyresearch.org/content/journal/micro/10.1099/mic.0.000265
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