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dc.contributor.author
Bejerman, Nicolas
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dc.contributor.author
Zanini, Andrea Alejandra
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dc.contributor.author
Rodríguez-Pardina, Patricia Elsa
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dc.contributor.author
Di Feo, Liliana del Valle
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dc.date.available
2022-11-30T18:15:54Z
dc.date.issued
2016-01
dc.identifier.citation
Bejerman, Nicolas; Zanini, Andrea Alejandra; Rodríguez-Pardina, Patricia Elsa; Di Feo, Liliana del Valle; Use of 454-Pyrosequencing for the Characterization of Sweet potato virus C and Sweet potato feathery mottle virus isolates from Argentina and development of a multiplex one-step RT-PCR for their simultaneous detection; Wiley Blackwell Publishing, Inc; Journal of Phytopathology-Phytopathologische Zeitschrift; 164; 6; 1-2016; 386-394
dc.identifier.issn
0931-1785
dc.identifier.uri
http://hdl.handle.net/11336/179614
dc.description.abstract
454-pyrosequencing was applied as a tool to identify etiological agents involved in the symptoms observed in a sweet potato sample from Argentina. RNA was purified from symptomatic material and sequenced using a Roche 454 GS-FLX+. BLAST analysis of the viral reads identified the presence of Sweet potato feathery mottle virus (SPFMV)-O and SPFMV-RC strains and Sweet potato virus C (SPVC). For SPFMV-O and SPFMV–RC, 10 878 and 10 812 nucleotides, respectively, were sequenced, whereas a sequence of 10 793 nucleotides was obtained for SPVC. Pairwise comparison of polyprotein nucleotide sequences of O and RC Argentinian isolates (Arg) showed 99% and 98.4% sequence identities with SPFMV-O and SPFMV-RC-M2-41, respectively, whereas SPVC-Arg showed 94.2%, 92.9% and 94.6% sequence identities with SPVC-Il, SPVC-C1 and SPVC-Bungo, respectively. These results allowed us to develop a one-step multiplex reverse transcription-polymerase chain reaction assay (mRT-PCR) for the simultaneous detection and differentiation of SPFMV-O-Arg and SPFMV-RC-Arg and SPVC-Arg. Three specific forward primers unique to each virus and one reverse primer based on a region conserved in all three viruses were designed and used in the assay. These primers were further evaluated using field samples collected from four provinces of Argentina. The results showed that SPVC was the most common virus in samples analysed. This study shows the usefulness of deep sequencing not only to rapidly identify mixed infections between SPFMV-O and SPFMV-RC and SPVC, but also to develop a reliable mRT-PCR assay for detection of these sweet potato pathogens, which cannot be discriminated by serological techniques.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Wiley Blackwell Publishing, Inc
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dc.rights
info:eu-repo/semantics/openAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
454-PYROSEQUENCING
dc.subject
MOLECULAR CHARACTERIZATION
dc.subject
MRT-PCR
dc.subject
POTYVIRUS
dc.subject
SWEET POTATO
dc.subject.classification
Agronomía, reproducción y protección de plantas
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dc.subject.classification
Agricultura, Silvicultura y Pesca
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dc.subject.classification
CIENCIAS AGRÍCOLAS
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dc.title
Use of 454-Pyrosequencing for the Characterization of Sweet potato virus C and Sweet potato feathery mottle virus isolates from Argentina and development of a multiplex one-step RT-PCR for their simultaneous detection
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2022-11-30T12:05:20Z
dc.journal.volume
164
dc.journal.number
6
dc.journal.pagination
386-394
dc.journal.pais
Alemania
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dc.journal.ciudad
Göttingen
dc.description.fil
Fil: Bejerman, Nicolas. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigaciones Agropecuarias. Instituto de Patología Vegetal; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
dc.description.fil
Fil: Zanini, Andrea Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
dc.description.fil
Fil: Rodríguez-Pardina, Patricia Elsa. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigaciones Agropecuarias. Instituto de Patología Vegetal; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
dc.description.fil
Fil: Di Feo, Liliana del Valle. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigaciones Agropecuarias. Instituto de Patología Vegetal; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
dc.journal.title
Journal of Phytopathology-Phytopathologische Zeitschrift
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dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1111/jph.12466
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://onlinelibrary.wiley.com/doi/10.1111/jph.12466
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