Artículo
Differential hormonal and gene expression dynamics in two inbred sunflower lines with contrasting dormancy level
Rosello, Paula Luciana
; Vigliocco, Ana Edit
; Andrade, Andrea Mariela
; Riera, Natalí Vanesa
; Calafat, Mario Jose
; Molas, María Lía
; Alemano, Sergio Gabriel
Fecha de publicación:
02/2016
Editorial:
Elsevier France-Editions Scientifiques Medicales Elsevier
Revista:
Plant Physiology and Biochemistry
ISSN:
0981-9428
Idioma:
Inglés
Tipo de recurso:
Artículo publicado
Clasificación temática:
Resumen
Seed germination and dormancy are tightly regulated by hormone metabolism and signaling pathway. We investigated the endogenous content of abscisic acid (ABA), its catabolites, and gibberellins (GAs), as well as the expression level of certain ABA and GAs metabolic and signaling genes in embryo of dry and imbibed cypselas of inbred sunflower (Helianthus annuus L., Asteraceae) lines: B123 (dormant) and B91 (non-dormant). Under our experimental conditions, the expression of RGL2 gene might be related to the ABA peak in B123 line at 3 h of imbibition. Indeed, RGL2 transcripts are absent in dry and early embedded cypselas of the non-dormant line B91. ABA increase was accompanied by a significant ABA-Glucosyl ester (ABA-GE) and phaseic acid (PA) (two ABA catabolites) decrease in B123 line (3 h) which indicates that ABA metabolism seems to be more active in this line, and that it would be involved in the imposition and maintenance of sunflower seed dormancy, as it has been reported for many species. Finally, an increase of bioactive GAs (GA1 and GA3) occurs at 12 h of imbibition in both lines after a decrease in ABA content. This study shows the first report about the RGL2 tissue-specific gene expression in sunflower inbred lines with contrasting dormancy level. Furthermore, our results provide evidence that ABA and GAs content and differential expression of metabolism and signaling genes would be interacting in seed dormancy regulation through a mechanism of action related to embryo itself.
Palabras clave:
DORMANCY
,
GENE EXPRESSION
,
GERMINATION
,
HELIANTHUS ANNUUS L.
,
PHYTOHORMONES
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Identificadores
Colecciones
Articulos(CCT - CORDOBA)
Articulos de CTRO.CIENTIFICO TECNOL.CONICET - CORDOBA
Articulos de CTRO.CIENTIFICO TECNOL.CONICET - CORDOBA
Citación
Rosello, Paula Luciana; Vigliocco, Ana Edit; Andrade, Andrea Mariela; Riera, Natalí Vanesa; Calafat, Mario Jose; et al.; Differential hormonal and gene expression dynamics in two inbred sunflower lines with contrasting dormancy level; Elsevier France-Editions Scientifiques Medicales Elsevier; Plant Physiology and Biochemistry; 102; 2-2016; 133-140
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