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dc.contributor.author
Borda, Enri Santiago
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dc.contributor.author
Reina, Silvia Lorena
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dc.contributor.author
Ganzinelli, Sabrina Belen
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dc.date.available
2022-11-25T17:09:45Z
dc.date.issued
2016-11
dc.identifier.citation
Borda, Enri Santiago; Reina, Silvia Lorena; Ganzinelli, Sabrina Belen; Role of Anti Muscarinic Acetylcholine IgA and anti autoantibodies in whole saliva from Primary Sjögren’s syndrome patients; Sci Forschen; International Journal of Dentistry and Oral Health; 2; 5; 11-2016; 123-129
dc.identifier.issn
2471-657X
dc.identifier.uri
http://hdl.handle.net/11336/179067
dc.description.abstract
Aims: The purpose of this report is to describe saliva IgA antibody against M3 muscarinic acetylcholine receptors (mAChRs) and anti-Ro autoantibodies in patients with primary Sjögren’s syndrome (pSS). In addition we want to clarify if this antibody anti-Ro is or not related to the presence of anti-IgA M3 mAChRs autoantibodies in whole saliva of pSS patients. Methods: Whole saliva samples were collected from healthy volunteers (n=30), patients with pSS anti-Ro positive (n=60) and patients with pSS anti-Ro negative (n=30). Saliva IgA patients and healthy subjects were tested by ELISA recognized the synthetic 25-mer peptide corresponding to the extracellular loop of the human M3 mAChRs. Also, concentration of nitrite/nitrate was determined by ELISA. Results: Optical density values for saliva IgA from pSS anti-Ro positive are significantly higher than those from IgA anti-Ro negative patients and IgA from normal subjects. These molecular interactions between IgA and human M3 mAChR synthetic peptide increased in optical density values compared with IgA from pSS anti-Ro negative and healthy subjects when M3 mAChR synthetic peptide was used as coating antigen. The specificity of this reaction was assessed by the ability of the M3 synthetic peptide (1x10-5 M) to inhibit the action when whole saliva was incubated previously with the M3 synthetic peptide for 40 min at 37ºC and then added together in the microtiter plates. On the other hand, the concentration of nitrate/nitrite in whole saliva was significantly decreased in pSS anti-Ro positive patients in comparison with those from IgA anti-Ro negative patients and healthy subjects. Conclusions: Patients presenting in saliva anti IgA anti-Ro positive are statistically significant in optical density values than those IgA from anti-Ro negative patients and healthy individuals. Also, the hypofunction of the salivary glands is associated with significant decrement of nitrate/nitrites levels in the saliva in pSS-Ro positive without any changes in pSS-Ro negative and healthy subjects.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Sci Forschen
dc.rights
info:eu-repo/semantics/openAccess
dc.rights.uri
https://creativecommons.org/licenses/by/2.5/ar/
dc.subject
IgA
dc.subject
Autoantibodies in sjögren syndrome
dc.subject
Saliva
dc.subject
Nitrites/nitrates
dc.subject.classification
Otras Ciencias Médicas
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dc.subject.classification
Otras Ciencias Médicas
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dc.subject.classification
CIENCIAS MÉDICAS Y DE LA SALUD
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dc.title
Role of Anti Muscarinic Acetylcholine IgA and anti autoantibodies in whole saliva from Primary Sjögren’s syndrome patients
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2022-11-25T12:04:24Z
dc.journal.volume
2
dc.journal.number
5
dc.journal.pagination
123-129
dc.journal.pais
Estados Unidos
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dc.journal.ciudad
Richmond
dc.description.fil
Fil: Borda, Enri Santiago. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Odontología. Cátedra de Farmacología; Argentina
dc.description.fil
Fil: Reina, Silvia Lorena. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Odontología. Cátedra de Farmacología; Argentina
dc.description.fil
Fil: Ganzinelli, Sabrina Belen. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Odontología. Cátedra de Farmacología; Argentina
dc.journal.title
International Journal of Dentistry and Oral Health
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://biocoreopen.org/ijdoh/Role-of-Anti-Muscarinic-Acetylcholine-Ig-and-anti-autoantibodies-in-whole-saliva-from-Primary-Sjogrens-syndrome-patients.php
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