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dc.contributor.author
Marin, Maia Solange
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dc.contributor.author
Holani, Ravi
dc.contributor.author
Blyth, Graham A. D.
dc.contributor.author
Drouin, Dominique
dc.contributor.author
Odeón, Anselmo Carlos
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dc.contributor.author
Cobo, Eduardo R.
dc.date.available
2022-11-18T17:03:06Z
dc.date.issued
2019-02
dc.identifier.citation
Marin, Maia Solange; Holani, Ravi; Blyth, Graham A. D.; Drouin, Dominique; Odeón, Anselmo Carlos; et al.; Human cathelicidin improves colonic epithelial defenses against Salmonella typhimurium by modulating bacterial invasion, TLR4 and pro-inflammatory cytokines; Springer; Cell and Tissue Research; 376; 2-2019; 433–442
dc.identifier.issn
0302-766X
dc.identifier.uri
http://hdl.handle.net/11336/178359
dc.description.abstract
The intestinal mucosa contributes to frontline gut defenses by forming a barrier (physical and biochemical) and preventing the entry of pathogenic microbes. One innate role of the human colonic epithelium is to secrete cathelicidin, a peptide with broad antimicrobial and immunomodulatory functions. In this study, the effect of cathelicidin in the maintenance of epithelial integrity, Toll-like receptor recognition, bacterial invasion and initiation of inflammatory response against Salmonella typhimurium is investigated in cultured human colonic epithelium. We found exogenous human cathelicidin restores the epithelial integrity in S. typhimurium-infected colonic epithelial (T84) cells by mostly post-translational effects associated with reorganization of zonula occludens (ZO)-1 tight junction proteins. Endogenous cathelicidin prevents S. typhimurium internalization as shown in colonic epithelial cells genetically deficient in the only human cathelicidin, LL-37 (shLL-37). Moreover, supplementation of shLL-37 cells with synthetic LL-37 reduces the grade of S. typhimurium internalization in a dose-dependent manner. Mechanistically, shLL-37 cells have lower gene expression of TLR4 and pro-inflammatory cytokine IL-1β in response to S. typhimurium. Thus, human cathelicidin aids in the early colonic epithelial defenses against enteric S. typhimurium by preventing bacterial invasion and maintaining epithelial barrier integrity, likely to occur due to the production of sensing TLR4 and pro-inflammatory cytokines.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Springer
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dc.rights
info:eu-repo/semantics/restrictedAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
ANTIMICROBIAL PEPTIDE
dc.subject
CATHELICIDIN
dc.subject
EPITHELIAL INTEGRITY
dc.subject
INNATE IMMUNITY
dc.subject
INTESTINAL EPITHELIUM
dc.subject
SALMONELLA TYPHIMURIUM
dc.subject.classification
Biología Celular, Microbiología
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dc.subject.classification
Ciencias Biológicas
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dc.subject.classification
CIENCIAS NATURALES Y EXACTAS
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dc.title
Human cathelicidin improves colonic epithelial defenses against Salmonella typhimurium by modulating bacterial invasion, TLR4 and pro-inflammatory cytokines
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2022-11-17T17:37:08Z
dc.journal.volume
376
dc.journal.pagination
433–442
dc.journal.pais
Alemania
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dc.journal.ciudad
Berlin
dc.description.fil
Fil: Marin, Maia Solange. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata; Argentina. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Buenos Aires Sur. Estación Experimental Agropecuaria Balcarce. Área de Investigación en Producción y Sanidad Animal; Argentina
dc.description.fil
Fil: Holani, Ravi. University of Calgary; Canadá
dc.description.fil
Fil: Blyth, Graham A. D.. University of Calgary; Canadá
dc.description.fil
Fil: Drouin, Dominique. University of Calgary; Canadá
dc.description.fil
Fil: Odeón, Anselmo Carlos. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Buenos Aires Sur. Estación Experimental Agropecuaria Balcarce. Área de Investigación en Producción y Sanidad Animal; Argentina
dc.description.fil
Fil: Cobo, Eduardo R.. University of Calgary; Canadá
dc.journal.title
Cell and Tissue Research
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dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://link.springer.com/article/10.1007/s00441-018-02984-7
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/https://doi.org/10.1007/s00441-018-02984-7
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