Mostrar el registro sencillo del ítem
dc.contributor.author
do Carmo, Fillipe L. R.
dc.contributor.author
Marques Da Silva, Wanderson
dc.contributor.author
Tavares, Guilherme C.
dc.contributor.author
Ibraim, Izabela C.
dc.contributor.author
Cordeiro, Barbara F.
dc.contributor.author
Oliveira, Emiliano R.
dc.contributor.author
Rabah, Houem
dc.contributor.author
Cauty, Chantal
dc.contributor.author
da Silva, Sara H.
dc.contributor.author
Canário Viana, Marcus V.
dc.contributor.author
Caetano, Ana C. B.
dc.contributor.author
dos Santos, Roselane G.
dc.contributor.author
de Oliveira Carvalho, Rodrigo D.
dc.contributor.author
Jardin, Julien
dc.contributor.author
Pereira, Felipe L.
dc.contributor.author
Folador, Edson L.
dc.contributor.author
Le Loir, Yves
dc.contributor.author
Figueiredo, Henrique C. P.
dc.contributor.author
Jan, Gwénaël
dc.contributor.author
Azevedo, Vasco
dc.date.available
2022-11-15T10:52:34Z
dc.date.issued
2018-08
dc.identifier.citation
do Carmo, Fillipe L. R.; Marques Da Silva, Wanderson; Tavares, Guilherme C.; Ibraim, Izabela C.; Cordeiro, Barbara F.; et al.; Mutation of the surface layer protein SlpB has pleiotropic effects in the probiotic propionibacterium freudenreichii CIRM-BIA 129; Frontiers Media; Frontiers in Microbiology; 9; AUG; 8-2018; 1-22
dc.identifier.issn
1664-302X
dc.identifier.uri
http://hdl.handle.net/11336/177751
dc.description.abstract
Propionibacterium freudenreichii is a beneficial Gram-positive bacterium, traditionally used as a cheese-ripening starter, and currently considered as an emerging probiotic. As an example, the P. freudenreichii CIRM-BIA 129 strain recently revealed promising immunomodulatory properties. Its consumption accordingly exerts healing effects in different animal models of colitis, suggesting a potent role in the context of inflammatory bowel diseases. This anti-inflammatory effect depends on surface layer proteins (SLPs). SLPs may be involved in key functions in probiotics, such as persistence within the gut, adhesion to host cells and mucus, or immunomodulation. Several SLPs coexist in P. freudenreichii CIRM-BIA 129 and mediate immunomodulation and adhesion. A mutant P. freudenreichii CIRM-BIA 129ΔslpB (CB129ΔslpB) strain was shown to exhibit decreased adhesion to intestinal epithelial cells. In the present study, we thoroughly analyzed the impact of this mutation on cellular properties. Firstly, we investigated alterations of surface properties in CB129ΔslpB. Surface extractable proteins, surface charges (ζ-potential) and surface hydrophobicity were affected by the mutation. Whole-cell proteomics, using high definition mass spectrometry, identified 1,288 quantifiable proteins in the wild-type strain, i.e., 53% of the theoretical proteome predicted according to P. freudenreichii CIRM-BIA 129 genome sequence. In the mutant strain, we detected 1,252 proteins, including 1,227 proteins in common with the wild-type strain. Comparative quantitative analysis revealed 97 proteins with significant differences between wild-type and mutant strains. These proteins are involved in various cellular process like signaling, metabolism, and DNA repair and replication. Finally, in silico analysis predicted that slpB gene is not part of an operon, thus not affecting the downstream genes after gene knockout. This study, in accordance with the various roles attributed in the literature to SLPs, revealed a pleiotropic effect of a single slpB mutation, in the probiotic P. freudenreichii. This suggests that SlpB may be at a central node of cellular processes and confirms that both nature and amount of SLPs, which are highly variable within the P. freudenreichii species, determine the probiotic abilities of strains.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Frontiers Media
dc.rights
info:eu-repo/semantics/openAccess
dc.rights.uri
https://creativecommons.org/licenses/by/2.5/ar/
dc.subject
BACTERIA GENOMIC
dc.subject
BACTERIA PROTEOMIC
dc.subject
HDMSE
dc.subject
SHOTGUN PROTEOMIC
dc.subject
SURFACE LAYER PROTEIN
dc.subject.classification
Biología Celular, Microbiología
dc.subject.classification
Ciencias Biológicas
dc.subject.classification
CIENCIAS NATURALES Y EXACTAS
dc.title
Mutation of the surface layer protein SlpB has pleiotropic effects in the probiotic propionibacterium freudenreichii CIRM-BIA 129
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2022-11-10T15:27:16Z
dc.journal.volume
9
dc.journal.number
AUG
dc.journal.pagination
1-22
dc.journal.pais
Suiza
dc.journal.ciudad
Lausanne
dc.description.fil
Fil: do Carmo, Fillipe L. R.. Institut National de la Recherche Agronomique; Francia. Universidade Federal de Minas Gerais. Instituto de Ciências Biológicas; Brasil
dc.description.fil
Fil: Marques Da Silva, Wanderson. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
dc.description.fil
Fil: Tavares, Guilherme C.. Universidade Federal de Minas Gerais; Brasil
dc.description.fil
Fil: Ibraim, Izabela C.. Universidade Federal de Minas Gerais. Instituto de Ciências Biológicas; Brasil
dc.description.fil
Fil: Cordeiro, Barbara F.. Universidade Federal de Minas Gerais. Instituto de Ciências Biológicas; Brasil
dc.description.fil
Fil: Oliveira, Emiliano R.. Universidade Federal de Minas Gerais. Instituto de Ciências Biológicas; Brasil
dc.description.fil
Fil: Rabah, Houem. Institut National de la Recherche Agronomique; Francia
dc.description.fil
Fil: Cauty, Chantal. Institut National de la Recherche Agronomique; Francia
dc.description.fil
Fil: da Silva, Sara H.. Universidade Federal de Minas Gerais. Instituto de Ciências Biológicas; Brasil
dc.description.fil
Fil: Canário Viana, Marcus V.. Universidade Federal de Minas Gerais. Instituto de Ciências Biológicas; Brasil
dc.description.fil
Fil: Caetano, Ana C. B.. Universidade Federal de Minas Gerais. Instituto de Ciências Biológicas; Brasil
dc.description.fil
Fil: dos Santos, Roselane G.. Universidade Federal de Minas Gerais. Instituto de Ciências Biológicas; Brasil
dc.description.fil
Fil: de Oliveira Carvalho, Rodrigo D.. Instituto de Ciencias Da Saúde; Brasil
dc.description.fil
Fil: Jardin, Julien. Institut National de la Recherche Agronomique; Francia
dc.description.fil
Fil: Pereira, Felipe L.. Universidade Federal de Minas Gerais; Brasil
dc.description.fil
Fil: Folador, Edson L.. Universidade Estadual da Paraiba; Brasil
dc.description.fil
Fil: Le Loir, Yves. Institut National de la Recherche Agronomique; Francia
dc.description.fil
Fil: Figueiredo, Henrique C. P.. Universidade Federal de Minas Gerais; Brasil
dc.description.fil
Fil: Jan, Gwénaël. Institut National de la Recherche Agronomique; Francia
dc.description.fil
Fil: Azevedo, Vasco. Universidade Federal de Minas Gerais. Instituto de Ciências Biológicas; Brasil
dc.journal.title
Frontiers in Microbiology
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://www.frontiersin.org/article/10.3389/fmicb.2018.01807/full
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.3389/fmicb.2018.01807
Archivos asociados