Artículo
Metabolic labeling of surface neo-sialylglyconjugates catalyzed by trypanosoma cruzi trans-sialidase
Carlevaro, Giannina Alejandra
; Lantos, Andrés Bernardo Gabriel
; Canepa, Gaspar Exequiel
; Camara, Maria de Los Milagros
; Somoza, Martín
; Buscaglia, Carlos Andres
; Campetella, Oscar Eduardo
; Mucci, Juan Sebastián
Fecha de publicación:
05/2019
Editorial:
Humana Press Inc.
Revista:
Methods in Molecular Biology
ISSN:
1064-3745
Idioma:
Inglés
Tipo de recurso:
Artículo publicado
Clasificación temática:
Resumen
Trypanosoma cruzi, the protozoan agent of Chagas disease, has evolved an innovative metabolic pathway by which protective sialic acid (SA) residues are scavenged from host sialylglycoconjugates and transferred onto parasite surface mucin-like molecules (or surface glycoconjugates from host target cells) by means of a unique trans-sialidase (TS) enzyme. TS-induced changes in the glycoprotein sialylation profile of both parasite and host cells are crucial for the establishment of a persistent T. cruzi infection and for the development of Chagas disease-associated pathogenesis. In this chapter, we describe a novel metabolic labeling method developed in our labs that enables straightforward identification and molecular characterization of SA acceptors of the TS-catalyzed reaction.
Palabras clave:
MUCINS
,
SIALIC ACID
,
SIALYLGLYCOCONJUGATES
,
TRANS-SIALIDASE
,
TRYPANOSOMA CRUZI
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Colecciones
Articulos (IIBIO)
Articulos de INSTITUTO DE INVESTIGACIONES BIOTECNOLOGICAS
Articulos de INSTITUTO DE INVESTIGACIONES BIOTECNOLOGICAS
Articulos(IIB-INTECH)
Articulos de INST.DE INVEST.BIOTECNOLOGICAS - INSTITUTO TECNOLOGICO CHASCOMUS
Articulos de INST.DE INVEST.BIOTECNOLOGICAS - INSTITUTO TECNOLOGICO CHASCOMUS
Citación
Carlevaro, Giannina Alejandra; Lantos, Andrés Bernardo Gabriel; Canepa, Gaspar Exequiel; Camara, Maria de Los Milagros; Somoza, Martín; et al.; Metabolic labeling of surface neo-sialylglyconjugates catalyzed by trypanosoma cruzi trans-sialidase; Humana Press Inc.; Methods in Molecular Biology; 1955; 5-2019; 135-146
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