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dc.contributor.author
Galera, Ivana Laura Delia
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dc.contributor.author
Páez, Paulina Laura
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dc.contributor.author
Paraje, María Gabriela
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dc.date.available
2022-11-08T17:37:06Z
dc.date.issued
2021
dc.identifier.citation
Antifungal activity of biogenic silver nanoparticles in combination with amphotericin B on Candida glabrata; LVII Annual Meeting of the Argentine Society for Biochemistry and Molecular Biology Research; XVI Annual Meeting of the Argentinean Society for General Microbiology; Argentina; 2021; 124-124
dc.identifier.issn
0327-9545
dc.identifier.uri
http://hdl.handle.net/11336/176910
dc.description.abstract
Non-albicans Candida (NAC) species cause 35-65% of all candidaemias in the general patient population. Among the NAC species, Candida glabrata is considered the second or third most frequent causative agent of superficial (oral, esophageal, urinary, and vaginal) or systemic infections, with a high mortality rate. These infections are difficult to treat for their innate resistance to many azole antifungals (ATF) therapy, especially fluconazole. Nowadays, current advances in nanotechnology constitute a promising alternative in the development of new antimicrobial agents. Silver nanoparticles (AgNPs) are very interesting products currently provided by available nanotechnology to evaluate their antifungal activity. In the present study, the synergism of AgNPs in combination with amphotericin B (AmB) against C. glabrata was investigated. Biogenic AgNPs were synthesized by eco-friendly method, and the antifungal activity against C. glabrata ATCC 2001 was evaluated through determination of Minimum Inhibitory Concentration (MIC50) and Minimum Fungicidal Concentration (MFC) according to protocol M27-A3 of Clinical and Laboratory Standards Institute (CLSI). The checkerboard microdilution method was used to study the synergistic combinations of AgNPs with AmB. The results were analyzed using the Fractionary Inhibitory Concentration (FIC) indices, a non-parametric model based on the Loewe additivity theory, and by CompuSyn software. CompuSyn is a computer program for quantitation of synergism and antagonism in drug combinations and the determination of IC50 (drug concentration causing 50% growth inhibition) and ED50 (dose causing 50% of maximum effect) values. Furthermore, we investigated the effects of the resazurin reduction (alamarBlue) assay, which measured metabolically active cells. The same MIC and MFC values were found for AgNP 0.13 pM and for AmB 2.7x105 pM. The FIC index was 0.37 (a FIC index of < 0.5 indicates synergism). This value corresponded to 0.033 pM AgNPs + 3.4 x 104 pM AmB (0.25 CIM AgNP + 0.125 CIM AmB) combination. Resazurin (blue, nonfluorescent) was reduced by metabolically active cells to resorufin (pink, fluorescent) showing the cytotoxic effect, which was visually weighted. The CompuSyn analysis confirmed synergism between biogenic AgNPs and AmB against C. glabrata. The analysis also shows that the maximal inhibitory activity of the combination is substantially expanded compared to those of the single agents. The IC50 was 0.08 CIM AgNPs and 0.06 CIM AmB (0.01 pM and 1.6x104 pM respectively). Isobolograms demonstrate a stark reduction of the AmB dose when used with AgNP to induce 50% inhibition or greater. Combined therapy has the advantage of attacking different targets by combining several active principles with different mechanisms of action. The development of new approaches has great clinical relevance in the treatment of mycoses.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Tech Science Press
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dc.rights
info:eu-repo/semantics/openAccess
dc.rights.uri
https://creativecommons.org/licenses/by/2.5/ar/
dc.subject
Antifungal activity
dc.subject
Biogenic nanoparticles
dc.subject
Silver nanoparticles
dc.subject
Candida
dc.subject.classification
Biología Celular, Microbiología
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dc.subject.classification
Ciencias Biológicas
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dc.subject.classification
CIENCIAS NATURALES Y EXACTAS
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dc.title
Antifungal activity of biogenic silver nanoparticles in combination with amphotericin B on Candida glabrata
dc.type
info:eu-repo/semantics/publishedVersion
dc.type
info:eu-repo/semantics/conferenceObject
dc.type
info:ar-repo/semantics/documento de conferencia
dc.date.updated
2022-09-22T10:23:46Z
dc.identifier.eissn
1667-5746
dc.journal.volume
46
dc.journal.number
1
dc.journal.pagination
124-124
dc.journal.pais
Estados Unidos
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dc.journal.ciudad
Henderson
dc.description.fil
Fil: Galera, Ivana Laura Delia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto Multidisciplinario de Biología Vegetal. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas Físicas y Naturales. Instituto Multidisciplinario de Biología Vegetal; Argentina
dc.description.fil
Fil: Páez, Paulina Laura. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Departamento de Farmacia; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Unidad de Investigación y Desarrollo en Tecnología Farmacéutica. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Unidad de Investigación y Desarrollo en Tecnología Farmacéutica; Argentina
dc.description.fil
Fil: Paraje, María Gabriela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto Multidisciplinario de Biología Vegetal. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas Físicas y Naturales. Instituto Multidisciplinario de Biología Vegetal; Argentina
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://www.techscience.com/biocell/v46nSuppl.1/46213
dc.conicet.rol
Autor
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dc.conicet.rol
Autor
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dc.conicet.rol
Autor
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dc.coverage
Nacional
dc.type.subtype
Congreso
dc.description.nombreEvento
LVII Annual Meeting of the Argentine Society for Biochemistry and Molecular Biology Research; XVI Annual Meeting of the Argentinean Society for General Microbiology
dc.date.evento
2021-11-01
dc.description.paisEvento
Argentina
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dc.type.publicacion
Journal
dc.description.institucionOrganizadora
Argentine Society for Biochemistry and Molecular Biology Research
dc.source.revista
Biocell
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dc.date.eventoHasta
2021-11-05
dc.type
Congreso
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