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dc.contributor.author
Malacari, Dario Amilcar
dc.contributor.author
Pécora, Andrea
dc.contributor.author
Pérez Aguirreburualde, Maria Sol
dc.contributor.author
Cardoso, Nancy Patricia
dc.contributor.author
Odeón, Anselmo Carlos
dc.contributor.author
Capozzo, Alejandra Victoria
dc.date.available
2022-11-03T19:44:40Z
dc.date.issued
2018-04
dc.identifier.citation
Malacari, Dario Amilcar; Pécora, Andrea; Pérez Aguirreburualde, Maria Sol; Cardoso, Nancy Patricia; Odeón, Anselmo Carlos; et al.; In vitro and in vivo characterization of a typical and a high pathogenic bovine viral Diarrhea virus type II strains; Frontiers Media; Frontiers in Veterinary Science; 5; 4-2018; 1-10
dc.identifier.uri
http://hdl.handle.net/11336/176330
dc.description.abstract
Non-cytopathic (ncp) type 2 bovine viral diarrhea virus (BVDV-2) is widely prevalent in Argentina causing high mortality rates in cattle herds. In this study, we characterized an Argentinean ncp BVDV-2 field isolate (98-124) compared to a high-virulence reference strain (NY-93), using in silico analysis, in vitro assays, and in vivo infections of colostrum-deprived calves (CDC) to compare pathogenic characters and virulence. In vitro infection of bovine peripheral blood mononuclear cells (PBMC) with BVDV 98-124 induced necrosis shortly after infection while NY-93 strain increased the apoptotic rate in infected cells. Experimental infection of CDC (n = 4 each) with these strains caused an enteric syndrome. High pyrexia was detected in both groups. Viremia and shedding were more prolonged in the CDC infected with the NY-93 strain. In addition, NY-93 infection elicited a severe lymphopenia that lasted for 14 days, whereas 98-124 strain reduced the leukocyte counts for 5 days. All infected animals had a diminished lymphoproliferation activity in response to a mitogen. Neutralizing and anti-NS3 antibodies were detected 3 weeks after infection in all infected calves. Virulence was associated with a more severe clinical score, prolonged immune-suppression, and a greater window for transmission. Studies of apoptosis/necrosis performed after in vitro PBMC infection also revealed differences between both strains that might be correlated to the in vivo pathogenesis. Our results identified 98-124 as a low-virulence strain.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Frontiers Media
dc.rights
info:eu-repo/semantics/openAccess
dc.rights.uri
https://creativecommons.org/licenses/by/2.5/ar/
dc.subject
APOPTOSIS
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BOVINE VIRAL DIARRHEA VIRUS
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COLOSTRUM-DEPRIVED CALVES
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IMMUNE-SUPPRESSION
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VIRULENCE
dc.subject.classification
Otras Ciencias Veterinarias
dc.subject.classification
Ciencias Veterinarias
dc.subject.classification
CIENCIAS AGRÍCOLAS
dc.title
In vitro and in vivo characterization of a typical and a high pathogenic bovine viral Diarrhea virus type II strains
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2022-11-03T12:55:22Z
dc.identifier.eissn
2297-1769
dc.journal.volume
5
dc.journal.pagination
1-10
dc.journal.pais
Suiza
dc.journal.ciudad
Lausana
dc.description.fil
Fil: Malacari, Dario Amilcar. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Virología; Argentina
dc.description.fil
Fil: Pécora, Andrea. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Virología; Argentina
dc.description.fil
Fil: Pérez Aguirreburualde, Maria Sol. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Patobiología; Argentina
dc.description.fil
Fil: Cardoso, Nancy Patricia. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Virología; Argentina
dc.description.fil
Fil: Odeón, Anselmo Carlos. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Buenos Aires Sur. Estación Experimental Agropecuaria Balcarce; Argentina
dc.description.fil
Fil: Capozzo, Alejandra Victoria. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Virología; Argentina
dc.journal.title
Frontiers in Veterinary Science
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/http://journal.frontiersin.org/article/10.3389/fvets.2018.00075/full
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.3389/fvets.2018.00075
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