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dc.contributor.author
Moras, Martina
dc.contributor.author
Hattab, Claude
dc.contributor.author
Gonzalez Menendez, Pedro
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Fader Kaiser, Claudio Marcelo
dc.contributor.author
Dussiot, Michael
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Larghero, Jerome
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Le Van Kim, Caroline
dc.contributor.author
Kinet, Sandrina
dc.contributor.author
Taylor, Naomi
dc.contributor.author
Lefevre, Sophie D.
dc.contributor.author
Ostuni, Mariano
dc.date.available
2022-10-25T18:49:26Z
dc.date.issued
2021-01
dc.identifier.citation
Moras, Martina; Hattab, Claude; Gonzalez Menendez, Pedro; Fader Kaiser, Claudio Marcelo; Dussiot, Michael; et al.; Human erythroid differentiation requires VDAC1-mediated mitochondrial clearance; Ferrata Storti Foundation; Haematologica; 107; 1; 1-2021; 167-177
dc.identifier.issn
0390-6078
dc.identifier.uri
http://hdl.handle.net/11336/174828
dc.description.abstract
Erythroblast maturation in mammals is dependent on organelle clearance throughout terminal erythropoiesis. We studied the role of the outer mitochondrial membrane protein voltage-dependent anion channel-1 (VDAC1) in human terminal erythropoiesis. We show that short hairpin (shRNA)-mediated downregulation of VDAC1 accelerates erythroblast maturation. Thereafter, erythroblasts are blocked at the orthochromatic stage, exhibiting a significant decreased level of enucleation, concomitant with an increased cell death. We demonstrate that mitochondria clearance starts at the transition from basophilic to polychromatic erythroblast, and that VDAC1 downregulation induces the mitochondrial retention. In damaged mitochondria from non-erythroid cells, VDAC1 was identified as a target for Parkin-mediated ubiquitination to recruit the phagophore. Here, we showed that VDAC1 is involved in phagophore’s membrane recruitment regulating selective mitophagy of still functional mitochondria from human erythroblasts. These findings demonstrate for the first time a crucial role for VDAC1 in human erythroblast terminal differentiation, regulating mitochondria clearance.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Ferrata Storti Foundation
dc.rights
info:eu-repo/semantics/openAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc/2.5/ar/
dc.subject
VDAC1
dc.subject
MITOPHAGY
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ERITROPOYESIS
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AUTOPHAGY
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Bioquímica y Biología Molecular
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Ciencias Biológicas
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CIENCIAS NATURALES Y EXACTAS
dc.title
Human erythroid differentiation requires VDAC1-mediated mitochondrial clearance
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2022-09-22T00:30:38Z
dc.identifier.eissn
1592-8721
dc.journal.volume
107
dc.journal.number
1
dc.journal.pagination
167-177
dc.journal.pais
Italia
dc.journal.ciudad
Pavia
dc.description.fil
Fil: Moras, Martina. Universite de Paris; Francia. Institut National de Transfusion Sanguine; Francia. Laboratoire d’Excellence GR-Ex; Francia
dc.description.fil
Fil: Hattab, Claude. Universite de Paris; Francia. Institut National de Transfusion Sanguine; Francia. Laboratoire d’Excellence GR-Ex; Francia
dc.description.fil
Fil: Gonzalez Menendez, Pedro. Laboratoire d’Excellence GR-Ex; Francia. Université Montpellier II; Francia. Centre National de la Recherche Scientifique; Francia
dc.description.fil
Fil: Fader Kaiser, Claudio Marcelo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos. Universidad Nacional de Cuyo. Facultad de Ciencias Médicas. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos; Argentina. Universidad Nacional de Cuyo. Facultad de Odontologia; Argentina
dc.description.fil
Fil: Dussiot, Michael. Universite de Paris; Francia. Laboratoire d’Excellence GR-Ex; Francia
dc.description.fil
Fil: Larghero, Jerome. Hôpital Saint-Louis. Unité de Thérapie cellulaire; Francia
dc.description.fil
Fil: Le Van Kim, Caroline. Universite de Paris; Francia. Institut National de Transfusion Sanguine; Francia. Laboratoire d’Excellence GR-Ex; Francia
dc.description.fil
Fil: Kinet, Sandrina. Laboratoire d’Excellence GR-Ex; Francia. Université Montpellier II; Francia. Centre National de la Recherche Scientifique; Francia
dc.description.fil
Fil: Taylor, Naomi. Laboratoire d’Excellence GR-Ex; Francia. Centre National de la Recherche Scientifique; Francia. Université Montpellier II; Francia. Center for Cancer Research; Estados Unidos
dc.description.fil
Fil: Lefevre, Sophie D.. Universite de Paris; Francia. Institut National de Transfusion Sanguine; Francia. Laboratoire d’Excellence GR-Ex; Francia
dc.description.fil
Fil: Ostuni, Mariano. Universite de Paris; Francia. Institut National de Transfusion Sanguine; Francia. Laboratoire d’Excellence GR-Ex; Francia
dc.journal.title
Haematologica
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://haematologica.org/article/view/haematol.2020.257121
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/https://doi.org/10.3324/haematol.2020.257121
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