Artículo
Detection of Echinococcus granulosus sensu lato infection by using extracts derived from a protoscoleces G1 cell line
Maglioco, Andrea Florencia
; Gentile, Jorge; Barbery Venturi, Melisa Silvana
; Jensen, Oscar; Hernández, Claudia; Gertiser, María Laura; Poggio, Thelma Veronica
; Canziani, Gabriela Alicia
; Fuchs, Alicia Graciela
Fecha de publicación:
09/2019
Editorial:
Wiley Blackwell Publishing, Inc
Revista:
Parasite Immunology
ISSN:
0141-9838
Idioma:
Inglés
Tipo de recurso:
Artículo publicado
Clasificación temática:
Resumen
Cystic echinococcosis (CE) can be diagnosed by means of several serological approaches, but their results vary among laboratories due to the molecular characteristics of the reference antigens used. Thus, this study aimed to address both the relevance of an EGPE cell line previously obtained from Echinococcus granulosus protoscoleces G1 and the complexity of the immune response by using two different in vitro growth stages as separate sources of parasite antigens. The serum reactivity was investigated by western blotting (WB) in 21 CE patients from an endemic area in a matched case-control design and also in seven experimentally infected sheep and five healthy control sheep. EGPE-antigen-human serum sensitivity by WB was higher than that of hydatid fluid (HF) WB, ELISA and DD5 (P <.05, Chi-square test). EGPE protein extract was immunogenic in mice and hyperimmune plasma reacted with HF proteins, and AgB2 expression was detected by molecular analysis. Proteins of 37 to 60 kDa were recognized by 95.24% of the CE patients’ sera but, with poor specificity. Statistically significant differences were found between serum protein extract recognition at 7 and 20 days of cell growth. The EGPE cell line is a laboratory source of antigens for improvement of CE serological diagnosis.
Palabras clave:
CELL LINE EXTRACT
,
CYSTIC ECHINOCOCCOSIS
,
HUMAN
,
IMMUNOBLOT
,
PARASITE
,
SERA
,
SHEEP
Archivos asociados
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Identificadores
Colecciones
Articulos(ICT - MILSTEIN)
Articulos de INST.DE CS. Y TECNOLOGIA "DR. CESAR MILSTEIN"
Articulos de INST.DE CS. Y TECNOLOGIA "DR. CESAR MILSTEIN"
Articulos(SEDE CENTRAL)
Articulos de SEDE CENTRAL
Articulos de SEDE CENTRAL
Citación
Maglioco, Andrea Florencia; Gentile, Jorge; Barbery Venturi, Melisa Silvana; Jensen, Oscar; Hernández, Claudia; et al.; Detection of Echinococcus granulosus sensu lato infection by using extracts derived from a protoscoleces G1 cell line; Wiley Blackwell Publishing, Inc; Parasite Immunology; 41; 12; 9-2019; 1-8
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