Artículo
Reconstruction and characterization of full-length begomovirus and alphasatellite genomes infecting pepper through metagenomics
Bornancini, Verónica Andrea
; Irazoqui, José Matías
; Flores, Ceferino René; Vaghi Medina, Carlos Gastón; Amadio, Ariel Fernando
; López Lambertini, Paola María
Fecha de publicación:
02/2020
Editorial:
Multidisciplinary Digital Publishing Institute
Revista:
Viruses
e-ISSN:
1999-4915
Idioma:
Inglés
Tipo de recurso:
Artículo publicado
Clasificación temática:
Resumen
In northwestern Argentina (NWA), pepper crops are threatened by the emergence of begomoviruses due to the spread of its vector, Bemisia tabaci (Gennadius). The genus Begomovirus includes pathogens that can have a monopartite or bipartite genome and are occasionally associated with sub-viral particles called satellites. This study characterized the diversity of begomovirus and alphasatellite species infecting pepper in NWA using a metagenomic approach. Using RCA-NGS (rolling circle amplification-next generation sequencing), 19 full-length begomovirus genomes (DNA-A and DNA-B) and one alphasatellite were assembled. This ecogenomic approach revealed six begomoviruses in single infections: soybean blistering mosaic virus (SbBMV), tomato yellow spot virus (ToYSV), tomato yellow vein streak virus (ToYVSV), tomato dwarf leaf virus (ToDfLV), sida golden mosaic Brazil virus (SiGMBRV), and a new proposed species, named pepper blistering leaf virus (PepBLV). SbBMV was the most frequently detected species, followed by ToYSV. Moreover, a new alphasatellite associated with ToYSV, named tomato yellow spot alphasatellite 2 (ToYSA-2), was reported for the first time in Argentina. For the Americas, this was the first report of an alphasatellite found in a crop (pepper) and in a weed (Leonurus japonicus). We also detected intra-species and inter-species recombination.
Palabras clave:
GEMINIALPHASATELLITINAE
,
GEMINIVIRIDAE
,
PEPPER
,
RCA-NGS
,
RECOMBINATION
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Articulos(CCT - SANTA FE)
Articulos de CTRO.CIENTIFICO TECNOL.CONICET - SANTA FE
Articulos de CTRO.CIENTIFICO TECNOL.CONICET - SANTA FE
Citación
Bornancini, Verónica Andrea; Irazoqui, José Matías; Flores, Ceferino René; Vaghi Medina, Carlos Gastón; Amadio, Ariel Fernando; et al.; Reconstruction and characterization of full-length begomovirus and alphasatellite genomes infecting pepper through metagenomics; Multidisciplinary Digital Publishing Institute; Viruses; 12; 2; 2-2020; 1-20
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