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dc.contributor.author
Ozcan, Selahattin C.
dc.contributor.author
Sarioglu, Aybike
dc.contributor.author
Altunok, Tugba H.
dc.contributor.author
Akkoc, Ahmet
dc.contributor.author
Guzel, Saime
dc.contributor.author
Guler, Sabire
dc.contributor.author
Imbert-Fernandez, Yoannis
dc.contributor.author
Muchut, Robertino José
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dc.contributor.author
Iglesias, Alberto Alvaro
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dc.contributor.author
Gurpinar, Yunus
dc.contributor.author
Clem, Amy L.
dc.contributor.author
Chesney, Jason A.
dc.contributor.author
Yalcin, Abdullah
dc.date.available
2022-10-24T11:31:20Z
dc.date.issued
2020-05
dc.identifier.citation
Ozcan, Selahattin C.; Sarioglu, Aybike; Altunok, Tugba H.; Akkoc, Ahmet; Guzel, Saime; et al.; PFKFB2 regulates glycolysis and proliferation in pancreatic cancer cells; Springer; Molecular and Cellular Biochemistry; 470; 1-2; 5-2020; 115-129
dc.identifier.issn
0300-8177
dc.identifier.uri
http://hdl.handle.net/11336/174483
dc.description.abstract
Tumor cells increase glucose metabolism through glycolysis and pentose phosphate pathways to meet the bioenergetic and biosynthetic demands of rapid cell proliferation. The family of 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatases (PFKFB1-4) are key regulators of glucose metabolism via their synthesis of fructose-2,6-bisphosphate (F2,6BP), a potent activator of glycolysis. Previous studies have reported the co-expression of PFKFB isozymes, as well as the mRNA splice variants of particular PFKFB isozymes, suggesting non-redundant functions. Majority of the evidence demonstrating a requirement for PFKFB activity in increased glycolysis and oncogenic properties in tumor cells comes from studies on PFKFB3 and PFKFB4 isozymes. In this study, we show that the PFKFB2 isozyme is expressed in tumor cell lines of various origin, overexpressed and localizes to the nucleus in pancreatic adenocarcinoma, relative to normal pancreatic tissue. We then demonstrate the differential intracellular localization of two PFKFB2 mRNA splice variants and that, when ectopically expressed, cytoplasmically localized mRNA splice variant causes a greater increase in F2,6BP which coincides with an increased glucose uptake, as compared with the mRNA splice variant localizing to the nucleus. We then show that PFKFB2 expression is required for steady-state F2,6BP levels, glycolytic activity, and proliferation of pancreatic adenocarcinoma cells. In conclusion, this study may provide a rationale for detailed investigation of PFKFB2’s requirement for the glycolytic and oncogenic phenotype of pancreatic adenocarcinoma cells.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Springer
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dc.rights
info:eu-repo/semantics/restrictedAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
FRUCTOSE-2,6-BISPHOSPHATE
dc.subject
GLYCOLYSIS
dc.subject
PANCREATIC ADENOCARCINOMA
dc.subject
PFKFB2
dc.subject.classification
Bioquímica y Biología Molecular
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dc.subject.classification
Ciencias Biológicas
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dc.subject.classification
CIENCIAS NATURALES Y EXACTAS
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dc.title
PFKFB2 regulates glycolysis and proliferation in pancreatic cancer cells
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2022-09-22T15:21:00Z
dc.journal.volume
470
dc.journal.number
1-2
dc.journal.pagination
115-129
dc.journal.pais
Alemania
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dc.journal.ciudad
Berlin
dc.description.fil
Fil: Ozcan, Selahattin C.. Koc University Research Center For Translational Medici; Turquía
dc.description.fil
Fil: Sarioglu, Aybike. Bursa Uludag University; Turquía
dc.description.fil
Fil: Altunok, Tugba H.. Bursa Uludag University; Turquía
dc.description.fil
Fil: Akkoc, Ahmet. Bursa Uludag University; Turquía
dc.description.fil
Fil: Guzel, Saime. Bursa Uludag University; Turquía
dc.description.fil
Fil: Guler, Sabire. Bursa Uludag University; Turquía
dc.description.fil
Fil: Imbert-Fernandez, Yoannis. University of Louisville; Estados Unidos
dc.description.fil
Fil: Muchut, Robertino José. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Agrobiotecnología del Litoral. Universidad Nacional del Litoral. Instituto de Agrobiotecnología del Litoral; Argentina
dc.description.fil
Fil: Iglesias, Alberto Alvaro. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Agrobiotecnología del Litoral. Universidad Nacional del Litoral. Instituto de Agrobiotecnología del Litoral; Argentina
dc.description.fil
Fil: Gurpinar, Yunus. Bursa Uludag University; Turquía
dc.description.fil
Fil: Clem, Amy L.. University of Louisville; Estados Unidos
dc.description.fil
Fil: Chesney, Jason A.. University of Louisville; Estados Unidos
dc.description.fil
Fil: Yalcin, Abdullah. Bursa Uludag University; Turquía
dc.journal.title
Molecular and Cellular Biochemistry
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dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/http://link.springer.com/10.1007/s11010-020-03751-5
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1007/s11010-020-03751-5
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