Artículo
14-3-3β isoform is specifically acetylated at Lys51 during differentiation to the osteogenic lineage
Frontini López, Yesica Romina
; Gojanovich, Aldana Daniela
; del Veliz, Samanta
; Uhart, Marina
; Bustos, Diego Martin
Fecha de publicación:
08/2021
Editorial:
Wiley-liss, div John Wiley & Sons Inc.
Revista:
Journal of Cellular Biochemistry
ISSN:
0730-2312
Idioma:
Inglés
Tipo de recurso:
Artículo publicado
Clasificación temática:
Resumen
The 14-3-3 protein family binds and regulates hundreds of serine/threonine phosphorylated proteins as an essential component of many signaling networks. Specific biological functions are currently been discovered for each of its seven isoforms in mammals. These proteins have been traditionally considered unregulated; however, its acetylation in an essential lysine residue, causing its inactivation, was recently published. Here, we studied the acetylation state of this lysine 49/51 during the osteogenic differentiation of human adipose-derived stem cells. We found that during this process, the levels of 14-3-3β (but not its isoform 14-3-3γ) acK49/51 increase, representing the first report linking this PTM to a specific isoform and a cellular process. Our results suggested that this posttranslational modification could be catalyzed by the HBO1 acetyltransferase, as overexpression of HBO1 increased specifically 14-3-3 acK49/51 acetylation. Acetylated 14-3-3 proteins are located primarily in the nucleus, where their active state has been described to bind H3 histones and many transcription factors. The inhibition of the expression of different isoforms showed that the specific silencing of the 14-3-3β gene, but not γ, increased significantly the osteogenic potential of the cells. This result correlated to the increase in acetylation of 14-3- 3β Lys 49/51 during osteogenesis. The possible role of this PTM in osteogenesis is discussed.
Palabras clave:
14-3-3 PROTEINS REGULATION
,
LYSINE ACETYLATION
,
OSTEOGENESIS
,
STEM CELLS
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Identificadores
Colecciones
Articulos(IHEM)
Articulos de INST. HISTOLOGIA Y EMBRIOLOGIA DE MEND DR.M.BURGOS
Articulos de INST. HISTOLOGIA Y EMBRIOLOGIA DE MEND DR.M.BURGOS
Citación
Frontini López, Yesica Romina; Gojanovich, Aldana Daniela; del Veliz, Samanta; Uhart, Marina; Bustos, Diego Martin; 14-3-3β isoform is specifically acetylated at Lys51 during differentiation to the osteogenic lineage; Wiley-liss, div John Wiley & Sons Inc.; Journal of Cellular Biochemistry; 122; 12; 8-2021; 1767-1780
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