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Saffioti, Nicolas Andres  
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Leal Denis, Maria Florencia  
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Herlax, Vanesa Silvana  
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Schwarzbaum, Pablo Julio  
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Pallarola, Diego Andres  
dc.date.available
2022-10-18T10:30:10Z  
dc.date.issued
2021  
dc.identifier.citation
A biomimetic device combining microfluidics with nanotechnology allows studying the adhesion of erythrocytes to blood vessels; 20th Internaitional Congress of the INternational Union for Pure Applied Biophysics; 50th Annual Meeting of the Brazilian Society for Biochemistry and Molecular Biology; 45th Congress of Brazilian Biophysics Society anda 13th Brazilian Society on Nuclear Biosciences Congress; Brasil; 2021; 1-2  
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http://hdl.handle.net/11336/173664  
dc.description.abstract
Erythrocytes under pathological conditions undergo eryptosis, a process characterized by biochemical and morphological changes such as phosphatidylserine (PS) exposure to the plasma membrane external layer. Eryptotic erythrocytes adhere to the endothelial cells (ECs), which may be important in the pathology of bacterial infections and congenital diseases like sickle cell disease. Externalized PS can bind to receptors expressed on ECs under pathological conditions. To understand the adhesion mechanism, we designed a device combining microfluidics with nanostructured surfaces to mimic the capillary architecture and the expression of adhesive molecules by the activated ECs. Microfluidic chips were prepared in PDMS using molds fabricated by photolithography. Nanostructured surfaces were synthesized by block copolymer lithography and consisted of a glass surface covered with 7 nm diameter gold nanoparticles (AuNPs), arranged in a quasi-hexagonal array. The microfluidic chip was adhered to the nanostructured surface by an O2 plasma treatment. The AuNPs were functionalized with a polyethylene glycol chain (PEG) that binds to the AuNPs by a thiol at one end and has a nitriloacetic group (NTA) at the other end. The NTA binds proteins expressing a His-Tag. The surface not occupied by AuNPs was covered with PLL-g-PEG. We corroborated the specific AuNPs functionalization by quartz microbalance and fluorescence microscopy using a GFP-His Tag. Then, we studied the erythrocytes adhesion to a device functionalized with Annexin V-His Tag at different flows. Two conditions that promote eryptosis, incubation at 50° C or treatment with ionomycin, significantly increased the adhesion of erythrocytes at flows up to 1.5 dyn/cm2, in comparison with untreated erythrocytes. However, eryptotic erythrocytes also showed adherence to a device functionalized with a PEG lacking NTA groups. This indicates that erythrocytes adhesion may not be mediated only by PS receptors. Future experiments will test the erythrocytes adhesion elicited by proteins usually expressed by the activated ECs.  
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application/pdf  
dc.language.iso
eng  
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Sociedade Brasileira de Bioquímica e Biología Molecular  
dc.rights
info:eu-repo/semantics/openAccess  
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/  
dc.subject
BIOMIMETIC  
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PHOSPHATIDYLSERINE  
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ENDOTHELIUM  
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Otras Ciencias Químicas  
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Ciencias Químicas  
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CIENCIAS NATURALES Y EXACTAS  
dc.title
A biomimetic device combining microfluidics with nanotechnology allows studying the adhesion of erythrocytes to blood vessels  
dc.type
info:eu-repo/semantics/publishedVersion  
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info:eu-repo/semantics/conferenceObject  
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info:ar-repo/semantics/documento de conferencia  
dc.date.updated
2022-09-22T11:43:21Z  
dc.journal.pagination
1-2  
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Brasil  
dc.journal.ciudad
San Pablo  
dc.description.fil
Fil: Saffioti, Nicolas Andres. Universidad Nacional de San Martin. Instituto de Nanosistemas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Química y Físico-Química Biológicas "Prof. Alejandro C. Paladini". Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Química y Físico-Química Biológicas; Argentina  
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Fil: Leal Denis, Maria Florencia. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Química y Físico-Química Biológicas "Prof. Alejandro C. Paladini". Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Química y Físico-Química Biológicas; Argentina  
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Fil: Herlax, Vanesa Silvana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Bioquímicas de La Plata "Prof. Dr. Rodolfo R. Brenner". Universidad Nacional de la Plata. Facultad de Ciencias Médicas. Instituto de Investigaciones Bioquímicas de La Plata "Prof. Dr. Rodolfo R. Brenner"; Argentina  
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Fil: Schwarzbaum, Pablo Julio. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Química y Físico-Química Biológicas "Prof. Alejandro C. Paladini". Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Química y Físico-Química Biológicas; Argentina  
dc.description.fil
Fil: Pallarola, Diego Andres. Universidad Nacional de San Martin. Instituto de Nanosistemas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina  
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/http://easyapp.ekmf.com.br/sg/uploads/eventos/evento8/documentos/abstract_book.pdf  
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Autor  
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Autor  
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Autor  
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Autor  
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dc.coverage
Internacional  
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Congreso  
dc.description.nombreEvento
20th Internaitional Congress of the INternational Union for Pure Applied Biophysics; 50th Annual Meeting of the Brazilian Society for Biochemistry and Molecular Biology; 45th Congress of Brazilian Biophysics Society anda 13th Brazilian Society on Nuclear Biosciences Congress  
dc.date.evento
2021-10  
dc.description.paisEvento
Brasil  
dc.type.publicacion
Book  
dc.description.institucionOrganizadora
Sociedade Brasileira de Bioquímica e Biología Molecular  
dc.source.libro
Programe and Abstract Book: 20th Internaitional Congress of the INternational Union for Pure Applied Biophysics; 50th Annual Meeting of the Brazilian Society for Biochemistry and Molecular Biology; 45th Congress of Brazilian Biophysics Society anda 13th Brazilian Society on Nuclear Biosciences Congress  
dc.type
Congreso