Artículo
In silico and in vitro Evaluation of Mimetic Peptides as Potential Antigen Candidates for Prophylaxis of Leishmaniosis
Guedes, Deborah Carbonera; Santiani, Manuel Hospinal; Carvalho, Joyce; Soccol, Carlos Ricardo; Minozzo, João Carlos; Machado de Ávila, Ricardo Andrez; de Moura, Juliana Ferreira; Ramos, Eliezer Lucas Pires; Castro, Guillermo Raul
; Chávez Olórtegi, Carlos; Thomaz Soccol, Vanete
![Icon](/themes/CONICETDigital/images/conicet.png)
Fecha de publicación:
01/2021
Editorial:
Frontiers Media S.A.
Revista:
Frontiers in Chemistry
e-ISSN:
2296-2646
Idioma:
Inglés
Tipo de recurso:
Artículo publicado
Clasificación temática:
Resumen
Antigen formulation is the main feature for the success of leishmaniosis diagnosis and vaccination, since the disease is caused by different parasite species that display particularities which determine their pathogenicity and virulence. It is desirable that the antigens are recognized by different antibodies and are immunogenic for almost all Leishmania species. To overcome this problem, we selected six potentially immunogenic peptides derived from Leishmania histones and parasite membrane molecules obtained by phage display or spot synthesis and entrapped in liposome structures. We used these peptides to immunize New Zealand rabbits and determine the immunogenic capacity of the chimeric antigen. The peptides induced the production of antibodies as a humoral immune response against L. braziliensis or L. infantum. Next, to evaluate the innate response to induce cellular activation, macrophages from the peptide mix-immunized rabbits were infected in vitro with L. braziliensis or L. infantum. The peptide mix generated the IFN-γ, IL-12, IL-4 and TGF-β that led to Th1 and Th2 cellular immune responses. Interestingly, this mix of peptides also induced high expression of iNOS. These results suggest that the mix of peptides derived from histone and parasites membrane molecules was able to mimic parasites proteins and induce cytokines important to CD4+ T cell Th1 and Th2 differentiation and effector molecule to control the parasite infection. Finally, this peptide induced an immune balance that is important to prevent immunopathological disorders, inflammatory reactions, and control the parasite infection.
Palabras clave:
CYTOKINES
,
IN VITRO INFECTION
,
LEISHMANIASIS
,
MIMETIC PEPTIDES
,
VACCINES
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Articulos(CINDEFI)
Articulos de CENT.DE INV EN FERMENTACIONES INDUSTRIALES (I)
Articulos de CENT.DE INV EN FERMENTACIONES INDUSTRIALES (I)
Citación
Guedes, Deborah Carbonera; Santiani, Manuel Hospinal; Carvalho, Joyce; Soccol, Carlos Ricardo; Minozzo, João Carlos; et al.; In silico and in vitro Evaluation of Mimetic Peptides as Potential Antigen Candidates for Prophylaxis of Leishmaniosis; Frontiers Media S.A.; Frontiers in Chemistry; 8; 1-2021; 6014091-6014100
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