Evento
Effect of 2’-nitroflavone on the expression of receptors associated with EGFR activity in breast cancer cells
Cebrón, Julieta Rocío; Bojorge, Mariana Andrea; Marder, Nora Mariel
; Miquet, Johanna Gabriela
; Gonzalez, Lorena
Tipo del evento:
Simposio
Nombre del evento:
Buenos Aires Breast Cancer Symposium
Fecha del evento:
17/05/2021
Institución Organizadora:
Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Fisiología, Biología Molecular y Neurociencias;
Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental;
Centro de Educaciones Médicas e Investigaciones Clínicas "Norberto Quirno";
Universidad Austral. Facultad de Ciencias Biomédicas. Instituto de Investigaciones en Medicina Traslacional;
Título de la revista:
Medicina (Buenos Aires)
Editorial:
Fundación Revista Medicina
ISSN:
1669-9106
Idioma:
Inglés
Clasificación temática:
Resumen
Flavonoids were proposed as chemopreventive and chemotherapeutic agents per se or in combination with traditional antitumoral drugs. Epidermal growth factor receptor (EGFR) is associated with tumorigenesis of several tissues and it can be involved in the molecular mechanism of action of several flavonoids. With the aim of investigating if a combinatory therapy involving 2'-nitroflavone (2'NF), a synthetic flavonoid with antitumor properties, and EGFRinhibitors would be a possible effective treatment for breast cancer, we studied if this flavone modulates the expression of EGFR or other receptors that interact or modulate EGFR activity. MDA-MB-231 and MCF-7 breast cancer cells were treated with 2´NF at different concentrations for48 h. Afterwards, the protein content of EGFR, ErbB2, ER alpha, Met and IGF-IR were assessed by immunoblotting. Besides, PARP cleavage and phosphorylation of p38 were determined in the same experimental conditions. Results showed that 2´NF produced a reduction on the content ofErbB2 and IGF-IR in MDA-MB-231 cells, while in MCF-7 cells a decrease on the expression of EGFR, ErbB2 and ER alpha was observed. Both cell lines presented an increment in p38 phosphorylation and PARP cleavage upon 2'NF treatment. In conclusion, 2'NF demonstrated to have effects on the expression of receptors associated with EGFR activity which could justify a possible combinatorytherapy involving 2'NF and EGFR inhibitors.
Palabras clave:
2?-nitroflavone
,
BREAST CANCER CELLS
,
EGFR
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Eventos(IQUIFIB)
Eventos de INST.DE QUIMICA Y FISICO-QUIMICA BIOLOGICAS "PROF. ALEJANDRO C. PALADINI"
Eventos de INST.DE QUIMICA Y FISICO-QUIMICA BIOLOGICAS "PROF. ALEJANDRO C. PALADINI"
Citación
Effect of 2’-nitroflavone on the expression of receptors associated with EGFR activity in breast cancer cells; Buenos Aires Breast Cancer Symposium; Argentina; 2021; 15-16
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