Artículo
Role of AMPK in the protective effects exerted by triiodothyronine in ischemic-reperfused myocardium
Hermann, Romina
; Mestre Cordero, Victoria Evangelina
; Fernández Pazos, María de Las Mercedes; Córdoba, Mailen Florencia; Reznik, Federico Joaquín; Vélez, Débora Elisabet; Fellet, Andrea L.
; Marina Prendes, María Gabriela
Fecha de publicación:
02/2021
Editorial:
BioScientifica
Revista:
Journal of Molecular Endocrinology
ISSN:
0952-5041
Idioma:
Inglés
Tipo de recurso:
Artículo publicado
Clasificación temática:
Resumen
Recent studies have provided evidence that triiodothyronine (T3) might play an effective role in the recovery of ischemic myocardium, through the preservation of mitochondrial function and the improvement of energy substrate metabolism. To this respect, it has been suggested that T3 could activate AMP-activated protein kinase (AMPK), the cellular ‘fuel-gauge’ enzyme, although its role has yet to be elucidated. The aim of the present study was to investigate the effects produced by acute treatment with T3 (60 nM) and the pharmacological inhibition of AMPK by compound C on isolated rat left atria subjected to 75 min simulated ischemia-75 min reperfusion. Results showed that T3 increased AMPK activation during simulated ischemia-reperfusion, while compound C prevented it. At the end of simulated reperfusion, acute T3 treatment increased contractile function recovery and cellular viability conservation. Mitochondrial ultrastructure was better preserved in the presence of T3 as well as mitochondrial ATP production rate and tissue ATP content. Calcium retention capacity, a parameter widely used as an indicator of the resistance of mitochondrial permeability transition pore (MPTP) to opening, and GSK-3β phosphorylation, a master switch enzyme that limits MPTP opening, were increased by T3 administration. All these beneficial effects exerted by T3 acute treatment were prevented when compound C was co-administrated. The present study provided original evidence that T3 enhances intrinsic activation of AMPK during myocardial ischemiareperfusion, being this enzyme involved, at least in part, in the protective effects exerted by T3, contributing to mitochondrial structure and function preservation, post-ischemic contractile recovery and conservation of cellular viability.
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Articulos(IQUIMEFA)
Articulos de INST.QUIMICA Y METABOLISMO DEL FARMACO (I)
Articulos de INST.QUIMICA Y METABOLISMO DEL FARMACO (I)
Citación
Hermann, Romina; Mestre Cordero, Victoria Evangelina; Fernández Pazos, María de Las Mercedes; Córdoba, Mailen Florencia; Reznik, Federico Joaquín; et al.; Role of AMPK in the protective effects exerted by triiodothyronine in ischemic-reperfused myocardium; BioScientifica; Journal of Molecular Endocrinology; 66; 3; 2-2021; 207-221
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