Artículo
Impact of high fat diet on the sterol regulatory element-binding protein 2 cholesterol pathway in the testicle
Funes, Abi Karenina
; Simón, Layla Yamila
; Colombo, Regina Lucía
; Avena Alvarez, María Virginia
; Monclus, Maria de Los Angeles
; Crescitelli, Julieta; Cabrillana, María Eugenia
; Conte, María Inés
; Cayado Gutiérrez, Niubys de Los Milagros
; Boarelli, Paola Vanina
; Fornes, Miguel Walter
; Saez Lancellotti, Tania Emilce Estefania
Fecha de publicación:
05/2021
Editorial:
Oxford University Press
Revista:
Molecular Human Reproduction
ISSN:
1360-9947
Idioma:
Inglés
Tipo de recurso:
Artículo publicado
Clasificación temática:
Resumen
Male fertility has been shown to be dependent on cholesterol homeostasis. This lipid is essential for testosterone synthesis and spermatogenesis, but its levels must be maintained in an optimal range for proper testicular function. In particular, sperm cells' development is very sensitive to high cholesterol levels, noticeably during acrosomal formation. The aim of this work was to study whether the molecular pathway that regulates intracellular cholesterol, the sterol regulatory element-binding protein (SREBP) pathway, is affected in the testicles of animals under a fat diet. To investigate this, we took advantage of the non-obese hypercholesterolemia (HC) model in New Zealand rabbits that displays poor sperm and seminal quality. The testicular expression of SREBP isoform 2 (SREBP2) and its target molecules 3-hydroxy-3-methyl-glutaryl-coenzyme A reductase (HMGCR) and low-density lipoprotein receptor (LDLR) were studied under acute (6months) and chronic (more than 12months) fat intake by RT-PCR, western blot and immunofluorescence. Our findings showed that fat consumption promoted down-regulation of the SREBP2 pathway in the testicle at 6months, but upregulation after a chronic period. This was consistent with load of testicular cholesterol, assessed by filipin staining. In conclusion, the intracellular pathway that regulates cholesterol levels in the testicle is sensitive to dietary fats, and behaves differently depending on the duration of consumption: it has a short-term protective effect, but became deregulated in the long term, ultimately leading to a detrimental situation. These results will contribute to the understanding of the basic mechanisms of the effect of fat consumption in humans with idiopathic infertility.
Palabras clave:
CHOLESTEROL
,
MALE INFERTILITY
,
SPERMATOZOA
,
SREBP
,
TESTICLE
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Articulos(IMBECU)
Articulos de INST. DE MEDICINA Y BIO. EXP. DE CUYO
Articulos de INST. DE MEDICINA Y BIO. EXP. DE CUYO
Citación
Funes, Abi Karenina; Simón, Layla Yamila; Colombo, Regina Lucía; Avena Alvarez, María Virginia; Monclus, Maria de Los Angeles; et al.; Impact of high fat diet on the sterol regulatory element-binding protein 2 cholesterol pathway in the testicle; Oxford University Press; Molecular Human Reproduction; 27; 5; 5-2021; 1-12
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