Artículo
A Dual Fluorescence–Spin Label Probe for Visualization and Quantification of Target Molecules in Tissue by Multiplexed FLIM–EPR Spectroscopy
Dong, Pin; Stellmacher, Johannes; Bouchet, Lydia María
; Nieke, Marius; Kumar, Amit; Osorio-Blanco, Ernesto R.; Nagel, Gregor; Lohan, Silke B.; Teutloff, Christian; Patzelt, Alexa; Schäfer Korting, Monika; Calderón, Marcelo; Meinke, Martina C.; Alexiev, Ulrike
![Icon](/themes/CONICETDigital/images/conicet.png)
Fecha de publicación:
06/2021
Editorial:
Wiley VCH Verlag
Revista:
Angewandte Chemie
ISSN:
1433-7851
e-ISSN:
1521-3773
Idioma:
Inglés
Tipo de recurso:
Artículo publicado
Clasificación temática:
Resumen
Simultaneous visualization and concentration quantification of molecules in biological tissue is an important though challenging goal. The advantages of fluorescence lifetime imaging microscopy (FLIM) for visualization, and electron paramagnetic resonance (EPR) spectroscopy for quantification are complementary. Their combination in a multiplexed approach promises a successful but ambitious strategy because of spin label-mediated fluorescence quenching. Here, we solved this problem and present the molecular design of a dual label (DL) compound comprising a highly fluorescent dye together with an EPR spin probe, which also renders the fluorescence lifetime to be concentration sensitive. The DL can easily be coupled to the biomolecule of choice, enabling in vivo and in vitro applications. This novel approach paves the way for elegant studies ranging from fundamental biological investigations to preclinical drug research, as shown in proof-of-principle penetration experiments in human skin ex vivo.
Archivos asociados
Licencia
Identificadores
Colecciones
Articulos(INFIQC)
Articulos de INST.DE INVESTIGACIONES EN FISICO- QUIMICA DE CORDOBA
Articulos de INST.DE INVESTIGACIONES EN FISICO- QUIMICA DE CORDOBA
Citación
Dong, Pin; Stellmacher, Johannes; Bouchet, Lydia María; Nieke, Marius; Kumar, Amit; et al.; A Dual Fluorescence–Spin Label Probe for Visualization and Quantification of Target Molecules in Tissue by Multiplexed FLIM–EPR Spectroscopy; Wiley VCH Verlag; Angewandte Chemie; 60; 27; 6-2021; 14938-14944
Compartir
Altmétricas