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Artículo

Retinal changes in an experimental model of early type 2 diabetes in rats characterized by non-fasting hyperglycemia

Salido, Ezequiel MartinIcon ; de Zavalia, Nuria Maria AsuncionIcon ; Schreier, Laura Ester; de Laurentiis, AndreaIcon ; Besuhli, ValeriaIcon ; Chianelli, Monica SilviaIcon ; Keller Sarmiento, Maria InesIcon ; Arias, Pablo; Rosenstein, Ruth EstelaIcon
Fecha de publicación: 04/2012
Editorial: Elsevier Inc
Revista: Experimental Neurology
ISSN: 0014-4886
Idioma: Inglés
Tipo de recurso: Artículo publicado
Clasificación temática:
Otras Ciencias de la Salud

Resumen

Diabetic retinopathy is a leading cause of acquired blindness in young, but also in elder adults, mostly affected by type 2 diabetes mellitus (T2DM). The aim of this work was to develop an experimental model of early human T2DM in adult rats, and to analyze retinal functional, morphological, and biochemical changes arising during the early stages of the moderate metabolic derangement. For this purpose, animals were divided in four groups: adult male Wistar rats receiving: tap water and citrate buffer i.p. (group 1), tap water with 30% sucrose and citrate buffer i.p. (group 2), tap water and 25 mg/kg i.p streptozotocin (STZ, group 3), or 30% sucrose and STZ (group 4). Fasting and postprandial glycemia, fructosamine and serum insulin levels were assessed. In addition, i.p. glucose and insulin tolerance tests were performed. Retinal function (electroretinogram, ERG) and morphology (optical microscopy), retinal nitric oxide synthase (NOS) activity (using 3H-arginine), lipid peroxidation (thiobarbituric acid reactive substances, TBARS), and TNFα levels (ELISA) were evaluated. At 6 and 12 weeks of treatment, animals which received a sucrose-enriched diet and STZ showed significant differences in most metabolic tests, as compared with the other groups. At 12 weeks of treatment, a significant decrease in the ERG a- and b- wave and oscillatory potential amplitudes, and a significant increase in retinal NOS activity, TBARS, TNFα, glial fibrillary acidic protein in Müller cells, and vascular endothelial growth factor levels were observed. These results indicate that the combination of diet-induced insulin resistance and a slight secretory impairment resulting from a low-dose STZ treatment mimics some features of human T2DM at its initial stages, and provokes significant retinal alterations.
Palabras clave: Type 2 Diabetes Mellitus , Experimental Model , Diabetic Retinopathy , Electroretinogram
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info:eu-repo/semantics/openAccess Excepto donde se diga explícitamente, este item se publica bajo la siguiente descripción: Atribución-NoComercial-SinDerivadas 2.5 Argentina (CC BY-NC-ND 2.5 AR)
Identificadores
URI: http://hdl.handle.net/11336/17255
URL: http://www.sciencedirect.com/science/article/pii/S0014488612001628
DOI: http://dx.doi.org/10.1016/j.expneurol.2012.04.010
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Articulos(CEFYBO)
Articulos de CENTRO DE ESTUDIOS FARMACOLOGICOS Y BOTANICOS
Citación
Salido, Ezequiel Martin; de Zavalia, Nuria Maria Asuncion; Schreier, Laura Ester; de Laurentiis, Andrea; Besuhli, Valeria; et al.; Retinal changes in an experimental model of early type 2 diabetes in rats characterized by non-fasting hyperglycemia; Elsevier Inc; Experimental Neurology; 236; 1; 4-2012; 151-160
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